活跃区成熟控制了前突触输出和释放模式,并由神经元活动调节
Yulia Akbergenova1,2, Jessica Matthias3, Sofya Makeyeva4,2
1Departments of Brain and Cognitive Sciences, The Picower Institute for Learning and Memory, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139 yulakb@mit.edu troy@mit.edu.
概括
突触成熟涉及到活跃区域 (AZ) 的蛋白质积累. 神经活动塑造了AZ的发展,影响了突触效率和神经递质在突触发育中的释放.
科学领域:
- 神经科学是一个神经科学.
- 细胞生物学 细胞生物学
- 发展生物学 发展生物学
背景情况:
- 突触形成需要突触前活性区 (AZ) 成熟,涉及细胞质蛋白和电压通道 (VGCC) 的有序组装.
- 幼虫Drosophila melanogaster神经肌肉结合点作为研究AZ成熟的动态过程及其功能后果的模型.
研究的目的:
- 为了研究活跃区域 (AZ) 成熟如何调节前突触输出和神经递质释放.
- 确定神经元活动在调节AZ成熟中的作用,包括支架蛋白质的结合和周转.
主要方法:
- 在时间印的Drosophila幼虫神经肌肉结节中AZ形成和功能的连续成像.
- 使用可光转化蛋白质 (mMaple,BRP,Unc13B,Unc13A) 来跟踪蛋白质动态和定位.
- 进行了量子成像,并分析了神经递质释放中断和基因突变 (rab3) 对AZ发育的影响.
主要成果:
- 与不成熟的突触相比,较老,更成熟的突触表现出更高的突触有效性和持续的神经递质释放.
- 减少的神经元活动减少了AZ播种,增加了现有AZ的物质积累,并减少了脚手架蛋白质周转.
- 活动减少影响了早期 (Unc13B) 和晚期 (Unc13A) 支架播种,通过一种独立于Rab3但依赖于 postsynaptic信号的机制.
结论:
- 活跃区域成熟是突触前释放模式和突触发育期间输出强度的关键调节者.
- 神经元活动在整个发育过程中积极塑造活跃区域的数量和大小,影响突触功能.
- 活动依赖的AZ成熟的调节涉及不同的机制,控制支架的整合和蛋白质周转.
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