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优化电用于有效生成对pyrifos的单克隆抗体,并在其应用在侧向流免疫试验

Tianyang You^1,2, Zhijia Wang^1,2, Shiyuan Yang^1,2

¹State Key Laboratory of Agricultural and Forestry Biosecurity, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China.

Journal of agricultural and food chemistry

|October 15, 2025

概括

研究人员开发了一种改进的电融合技术,以制造高质量的单克隆抗体 (mAbs),用于检测pyrifos农药残留物. 这导致了农产品基于黄金纳米粒子的快速和灵敏的侧流免疫试验 (AuNP-LFIA).

科学领域:

农业化学农业化学
免疫技术是一种免疫技术.
分析化学分析化学

背景情况:

免疫测试对于检测农药残留物至关重要,抗体性能是关键.
pyrifos (CHP) 是一种广泛使用的杀虫剂,需要可靠的检测方法.

研究的目的:

为了优化电融合,产生高亲和度的单克隆抗体 (mAbs) 对pyrifos.
开发一种基于黄金纳米粒子的灵敏,快速的侧流免疫试验 (AuNP-LFIA),用于检测pyrifos残留物.

主要方法:

通过优化电融合协议,合成了四种哈普,并产生了17mAbs的抗pyrifos.
通过B淋巴细胞的负分类和特定诱导剂治疗,提高电率至0.206%.
使用mAb 7A2F8E5开发了一个AuNP-LFIA,并通过UPLC-MS/MS验证了它的准确性.

主要成果:

与传统方法相比,电速率提高了10倍.
开发的AuNP-LFIA显示了缓冲中CHP的1.25 ng/mL的视觉检测极限.
在作物中检测到0.0050.02毫克/公斤的CHP残留物,低于国际最大残留物限值.

结论:

该研究提出了一种新且高效的电融合策略,用于生产高亲密度的mAbs.
建立了一个快速,灵敏和可靠的AuNP-LFIA用于实际监测农产品中的pyrifos残留物.

关键词:

pyrifosos 是一种酸盐.电融合电气是如何实现的诱导器诱导器的诱导器侧流免疫测试是一种侧流免疫测试.这是一种单克隆抗体.

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