通过UFLC/QTRAP-MS和PCA对来自不同地区的Gardenia jasminoides中生物活性化合物的分析
Chuan Chai1, Bo Jin2, Yuhan Cui1
1School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, Jiangsu, China.
Medicine
|October 15, 2025
概括
这项研究开发了一种分析Gardenia jasminoides Ellis (GJE) 质量的方法,揭示了其生物活性化合物的显著区域差异. 来自 Jurong 城市的 GJE 显示了更高的成分水平,为传统中医标准化提供了见解.
科学领域:
- 药理学是指药理学,即药理学是指药理学.
- 分析化学 分析化学
- 传统中国医药 传统中国医药
背景情况:
- 果园莉花Ellis (GJE) 是一种重要的传统中医药,具有成熟的治疗用途.
- 以前对GJE的质量评估往往侧重于单个化合物,缺乏全面的分析和地理相关性.
- 了解GJE化学成分的区域差异对于确保一致的治疗疗效和质量标准化至关重要.
研究的目的:
- 开发和验证一种超快的液体染色学-三重四极质谱法 (UFLC-TQMS) 方法,用于同时确定GJE中的多种生物活性化合物.
- 调查来自不同地区的GJE样本化学组成的地理差异.
- 为全面的GJE质量评估和区域差异化建立一个强大的分析方法.
主要方法:
- 从不同地理位置收集了18批GJE样本.
- 用UFLC-TQMS在21个目标化合物的多重反应监测模式下分析的样本.
- 使用主要成分分析 (PCA) 来评估区域化学成分差异并验证分析方法.
主要成果:
- UFLC-TQMS方法证明了21种目标化合物的优异的线性,精度,回收和稳定性.
- 总体化糖类,总体黄类和总体酸的含量存在显著的区域差异.
- 来自江苏省朱龙市的GJE样本显示出高水平的目标成分,PCA证实了这一点 (P < .05).
结论:
- 开发的UFLC-TQMS方法为全面的GJE质量评估和区域变异分析提供了可靠的工具.
- 地理来源是GJE化学特征和生物活性化合物含量的重要决定因素.
- 这种多元组件分析方法为GJE质量标准化,种植地点选择和TCM行业的收获优化提供了实际框架.
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