通过路径和过程优化,在Bacillus subtilis中增强了微生物的素 (维生素B6) 生产
Ai-Tong Jiang1,2,3,4, Guang-Qing Du2,3,4, Xu-Yang Huang1,2,3,4
1School of Biological Engineering, Dalian Polytechnic University, Dalian, China.
Synthetic and systems biotechnology
|October 15, 2025
概括
这项研究通过基因改造和优化发酵,对细菌进行了改造,使其产生更多的维生素B6的一种形式-pyridoxine (PN),通过基因改造和优化发酵. 这种改造品种取得了创纪录的PN产量174.6mg/L.
科学领域:
- 生物技术是生物技术.
- 微生物工程 微生物工程
- 代谢工程是代谢工程.
背景情况:
- 维生素B6,特别是素 (PN),对于许多生理过程至关重要.
- 微生物发酵为PN生物合成提供了一个可持续的途径,但天然菌株的低产量阻碍了工业应用.
- 提高微生物系统中的PN产量对于满足需求至关重要.
研究的目的:
- 使用 Bacillus subtilis. 开发一种高产的素 (PN) 菌株.
- 通过路径工程和发酵过程优化相结合,改善PN生产.
- 在B. subtilis.中建立PN生物合成的新纪录.
主要方法:
- 过度表达的关键基因 (epd,pdxB,serC,pdxA,pdxJ) 在脱氧化-5-酸盐 (DXP) 依赖的途径.
- 对DXP独立通路基因 (pdxST) 的核糖体结合部位 (RBS) 序列的选和优化.
- 系统地优化介质和料批发发酵,以增强PN生物合成.
主要成果:
- 过度表达DXP依赖的通路基因增加了PN标位的3.2倍,达到2.9 mg/L.
- 对DXP独立路径基因和RBS序列的优化进一步提高了PN标位,达到24.6 mg/L.
- 中等优化和料批发发酵导致B. subtilis. 的PN标位达到创纪录的174.6 mg/L.
结论:
- 路径工程和媒介优化是显著改善PN生产的有效策略.
- 开发的高产B. subtilis菌株为工业PN生物合成提供了一个有前途的平台.
- 这项研究为通过微生物发酵大规模生产维生素B6提供了宝贵的见解.
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