相关实验视频

Updated: Jan 15, 2026

In Vivo Nanovector Delivery of a Heart-specific MicroRNA-sponge

In Vivo Nanovector Delivery of a Heart-specific MicroRNA-sponge

Published on: June 15, 2018

微RNA-21通过向Smad-7来保护缺氧诱导的心肌细胞损伤

Md Sayed Ali Sheikh¹, A Alduraywish¹, Basil Mohammed Alomair¹

¹Internal Medicine Department, College of Medicine, Jouf University, Sakaka 72345, Saudi Arabia.

|October 15, 2025

概括

微RNAmiR-21,miR-488和miR-126显示了急性心肌梗塞 (AMI) 的诊断潜力. 通过减少细胞损伤,抑制miR-21可能为AMI提供治疗点.

科学领域:

心血管研究研究心血管研究
分子生物学分子生物学
生物标志物发现发现

背景情况:

急性心肌梗塞 (AMI) 是全球突然心脏死亡的主要原因.
了解AMI的分子机制和诊断标记对于改善患者的治疗结果至关重要.

研究的目的:

在急性心肌梗塞 (AMI) 患者中研究血miR-21,miR-488和miR-126的诊断作用.
探索miR-21抑制对H9c2细胞中缺氧诱导的细胞损伤的分子影响.
评估这些microRNAs作为AMI临床生物标志物的潜力.

主要方法:

对95名非ST升高心肌梗塞 (NSTEMI) 患者,152名ST升高心肌梗塞 (STEMI) 患者和95名健康对照者的血微RNA水平的分析.
用了三个月大的小鼠和心室衍生的H9c2细胞进行细胞研究.
用受体操作特征 (ROC) 曲线分析用于诊断评估和在H9c2细胞上使用miR-21抑制剂的体外实验.

主要成果:

血miR-21和miR-488水平显著上调,而miR-126水平在NSTEMI和STEMI患者下调,与健康个体相比.
ROC曲线分析证实了这些microRNAs在区分AMI患者与对照者的诊断能力.
在H9c2细胞中抑制miR-21减少了活性氧物种 (ROS),酶-3活性和亡,同时通过上调Smad-7表达来增强活力.

结论:

循环中的miR-21,miR-488和miR-126是诊断AMI的潜在生物标志物.
抑制miR-21通过针对Smad-7缓解缺氧诱导的细胞损伤,为AMI提供了一个有希望的治疗策略.
老年AMI患者血miR-21水平升高需要进一步调查,而低MIR-21可能会影响衰老过程.

关键词:

细胞中的H9c2细胞.这就是Smad-7的意义.剧烈的心肌梗塞急性心肌梗塞老化的老化衰老的老化生物标志物生物标志物微RNAs 是一个微型RNA.

更多相关视频

In vitro Assessment of Myocardial Protection following Hypothermia-Preconditioning in a Human Cardiac Myocytes Model

In vitro Assessment of Myocardial Protection following Hypothermia-Preconditioning in a Human Cardiac Myocytes Model

Published on: October 27, 2020

Tissue-specific miRNA Expression Profiling in Mouse Heart Sections Using In Situ Hybridization

Tissue-specific miRNA Expression Profiling in Mouse Heart Sections Using In Situ Hybridization

Published on: September 15, 2018

相关概念视频

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...