一个"打开"的 (III) 复杂探针用于可视化癌细胞和瘤模型中的线粒体SO2波动
Si-Yu Chen1,2, Meng-Sen Chen1, An-Li Liu1,2
1College of Chemistry and Chemical Engineering, Hainan Normal University, Haikou 571158, China. niuzhigang1982@126.com.
Dalton transactions (Cambridge, England : 2003)
|October 15, 2025
概括
一个新的 (III) 复合物探头,Ir-CHO,使灵敏和有选择性的探头成为可能.
科学领域:
- 生物医学工程 生物医学工程
- 化学生物学 化学生物学
- 分子成像学分子成像学
背景情况:
- 线粒体二氧化硫 (SO2) 对生理过程至关重要.
- 改变的SO2水平是早期瘤诊断的潜在生物标志物.
- 在癌细胞和瘤中可视化SO2对于研究至关重要.
研究的目的:
- 设计和合成一个"启动"的 (III) 复合探头 (Ir-CHO) 用于SO2检测.
- 评估探测器在活细胞和瘤模型中的SO2生物成像中的有效性.
- 评估探针在诊断瘤和监测瘤微环境方面的潜力.
主要方法:
- 使用ppy和PIP-CHO连接物合成一种 (III) 复合探针 (Ir-CHO).
- 探测器光物理性质的表征 (量子产量,寿命,检测极限).
- 在HeLa细胞和瘤携带小鼠中对SO2进行生物成像的探针的应用.
主要成果:
- 伊尔-CHO探测器展示了高光量子产量,微秒寿命和低检测极限.
- 探测器表现出快速反应,优异的光稳定性,选择性,特异性和线粒体准.
- 在活细胞中,Ir-CHO成功地可视化了内源/外源SO2,并将瘤与正常小鼠区分开来.
结论:
- 瘤微环境中的SO2实时监测的Ir-CHO探测器是一个有前途的工具.
- 由于其低细胞毒性,该探针显示出早期瘤诊断和生物医学应用的潜力.
- 这项工作强调了SO2监测在癌症研究和临床环境中的诊断潜力.
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