评价VITEK 2和三项直接敏感性测试的协议,用于从阳性血液培养瓶中检测出格拉姆阴性细菌
Özlem Aydemir1,2, Belgin Eda Misi3,4, Hatice Erdal3,4
1Sakarya University Faculty of Medicine Department of Medical Microbiology, Sakarya, Turkey. ozlemaydemir@sakarya.edu.tr.
概括
使用VITEK 2的阳性血液培养的直接抗生素敏感性测试 (AST) 提供了高准确度的格拉姆阴性细菌. 这种方法可以更快地给予抗微生物药物,改善血液感染患者的治疗结果.
科学领域:
- 临床微生物学 临床微生物学
- 传染病 传染病 传染病
- 抗微生物耐药性 抗微生物耐药性
背景情况:
- 血流感染 (BSI) 是死亡的主要原因之一.
- 早期和适当的抗生素治疗对于减少BSI相关死亡至关重要.
- 从阳性血液培养中直接进行抗生素敏感性测试 (AST) 可以加快与常规方法相比,抗菌剂的施用.
研究的目的:
- 通过使用阳性血培液,评估三种直接的AST协议的Gram-阴性细菌 (GNB) 的性能.
- 将直接的AST方法的准确性与参考标准进行比较.
主要方法:
- AST是在使用VITEK 2作为参考方法 (协议1) 对亚培养分离物进行的.
- 从阳性血液培养瓶中测试了三个直接的AST协议:VITEK 2 (协议2),修改的磁盘扩散 (协议3) 和快速磁盘扩散 (协议4).
- 2,3,4号协议的结果与1号协议进行了比较.
主要成果:
- 维特克2直接AST (协议2) 与参考方法达成了95.9%的分类一致 (CA).
- 经过修改的磁盘扩散 (协议3) 和快速磁盘扩散 (协议4) 显示较低的CA分别为80.7%和80.6%.
- 协议2显示了最低的非常重大差异 (0.5%) 和重大差异 (2.5%) 率.
结论:
- 从血液培养瓶中使用VITEK 2的直接AST为GNB提供了最高的CA和最低的差异率.
- 这种方法适用于具有质谱学能力的临床微生物学实验室.
- 直接AST提供了一种具有成本效益的方法,用于在BSI中及时选择抗菌剂.
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