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相关概念视频

Phase Contrast and Differential Interference Contrast Microscopy01:26

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Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
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Digital Inline Holographic Microscopy DIHM of Weakly-scattering Subjects
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通过散射层进行运动知情广场动态成像.

Haiming Yuan, Fei Wang, Guohai Situ

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    此摘要是机器生成的。

    定位和流融合 (LS-Fusion) 能够通过散射介质对移动物体进行广场光学成像. 这种方法可以在没有复杂设备的情况下,以高率实现大视野.

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    科学领域:

    • 光学成像技术的使用.
    • 生物医学光学 生物医学光学
    • 分散介质成像的成像.

    背景情况:

    • 深度光学成像至关重要,但受到散射的阻碍.
    • 现有的方法在速度,视野 (FOV) 或复杂性方面存在局限性.
    • 特殊的样本要求往往限制了实际应用.

    研究的目的:

    • 通过散射介质引入一种用于通过散射介质对移动物体进行广场成像的新方法.
    • 为了克服与当前深度光学成像技术相关的权衡.
    • 通过简单的实验设置实现高质量的动态成像.

    主要方法:

    • 拟议的定位和流融合 (LS-Fusion) 技术.
    • 处理对象运动作为非负矩阵因子化 (NMF) 的等效调制.
    • 共同重建的视频序列用于增强的源恢复.

    主要成果:

    • 在本机相机的率下使用散射光实现了大FOV成像.
    • 在没有专门的调制设备的情况下启用成像.
    • 在小鼠大脑切片中显示的FOV比记忆效应范围 (MER) 大5.7倍.
    • 启用了以每秒50 (fps) 的动态成像.

    结论:

    • 通过散射介质进行深度光学成像,LS-Fusion提供了一种简单而有效的解决方案.
    • 该方法成功地拍摄了超出传统NMF界限的距离很远的点源.
    • 在复杂的生物样本中,LS-Fusion可提供高质量,高速的动态成像.