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在山羊 (Capra hircus) 植入不同阶段的动态子宫内膜变化的综合转录基因分析

Junyin Zhao¹, Shengnan Zhao¹, Yangshen Zhang¹

¹Chongqing Key Laboratory of Herbivore Science, College of Animal Science and Technology, Southwest University, Chongqing 400715, China.

Journal of animal science

|October 15, 2025

概括

这项研究揭示了关键的非编码RNA和mRNA,这些RNA对山羊胚胎植入过程中的子宫内膜受容性至关重要. 了解这些分子调节剂可以更好地了解反动物成功怀孕的结果.

科学领域:

生殖生物学生殖生物学
基因组学就是基因组学.
分子内分泌学分子内分泌学

背景情况:

子宫内膜受容性对于胚胎植入和成功怀孕至关重要.
非编码RNAs (ncRNAs) 和信使RNAs (mRNAs) 在调节子宫功能方面起着复杂的作用.
描述这些分子动态对于理解牲畜的繁殖成功至关重要.

研究的目的:

在关键的植入阶段,系统地描述山羊子宫内膜中ncRNA和mRNA的动态格局.
确定关键的分子参与者和参与建立子宫内膜受体性的调节途径.
通过竞争的内源性RNA (ceRNA) 网络探索ncRNAs和mRNAs之间的潜在相互作用.

主要方法:

在妊娠第10天和第17天的山羊子宫内膜组织的转录组测序 (mRNA,lncRNA,circRNA,miRNA) 和在妊娠第17天的妊娠失败.
在不同生殖阶段对差异表达的基因和RNA的分析.
功能性丰富分析和ceRNA网络的构建.

主要成果:

在植入阶段识别了成千上万种差异表达的mRNA,lncRNA,circRNA和miRNA.
突出了MAPK,TNF和RAS信号通路在子宫内膜受体性中的参与.
发现了特定的ncRNAs (例如MSTRG774.1, novel_circ_000145) 和枢纽基因 (例如ISG15,CXCL10),这些基因对子宫受体性至关重要.

结论:

这项研究阐明了控制山羊子宫内膜接受性的复杂分子网络.
确定了新的ncRNA和mRNA,它们在胚胎植入过程中具有潜在的调节作用.
提供了关于反动物繁殖成功的分子机制的基础见解.

关键词:

羊 (Capra hircus) 是一个有趣的动物.胚胎植入胚胎的植入宫内膜的受体性宫内膜的受体性在 ncRNA 调节过程中.转录组生物标志物转录组生物标志物

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