相关实验视频
Updated: Jan 15, 2026
07:38
Measuring In Vitro ATPase Activity for Enzymatic Characterization
Published on: August 23, 2016
18.9K
重新审视环境 蛋白质的特异性 (脱溶) ATP的溶解能力
Indrani Bhattacharya1, Asesh Bera1, Aritra Marick1
1Department of Chemical and Biological Sciences, S. N. Bose National Centre for Basic Sciences, Block-JD, Sector-III, Salt Lake, Kolkata 700106, India.
Langmuir : the ACS journal of surfaces and colloids
|October 15, 2025
概括
在特定的酸性条件下,腺三酸盐 (ATP) 可以矛盾地诱导蛋白质纤维化,作为聚合媒介而不是水管. 这挑战了它在溶解蛋白质中的普遍作用.
科学领域:
- 生物化学 生化学
- 生物物理学的生物物理.
- 材料科学 材料科学 材料科学
背景情况:
- 腺三酸盐 (ATP) 通常被认为是一种生物水管,在细胞压力下帮助蛋白质溶解.
- 在蛋白质溶解性和聚合中ATP的普遍作用需要在不同的环境条件下仔细检查.
研究的目的:
- 在特定环境条件下,研究在ATP的存在下球状蛋白 (BSA和卵蛋白) 的聚合行为.
- 为了确定ATP是否普遍充当蛋白质溶解剂,或者它是否可以调节聚合.
主要方法:
- 在环境温度和pH值下进行蛋白纤维化实验 2.
- 使用场发射扫描电子显微镜 (FESEM),高分辨率传输电子显微镜 (HRTEM) 和循环二元化 (CD) 的聚合物的表征.
- 使用特拉赫兹-弗里耶变换红外光谱法 (THz-FTIR) 估计蛋白质水合和ATP-蛋白相互作用.
主要成果:
- 在pH 2和25°C时,ATP诱导牛血清白蛋白 (BSA) 和卵蛋白 (Ova) 的瞬间纤维化,形成粉样纤维.
- 这种纤维化与ATP抑制热展开的已知能力形成鲜明对比.
- THz-FTIR证实了ATP的三酸盐部分与暴露于溶剂的充电残留物结合,从而启动聚合.
- 聚合动力学取决于蛋白质残留成分,富含氨酸的BSA表现出比卵蛋白更快的纤维化.
结论:
- ATP的作用取决于上下文;它可以充当生物聚合媒介,而不仅仅是水管.
- 环境条件显著影响ATP对蛋白质结构和聚合的影响.
- 蛋白质残留的特异性,特别是素含量,影响聚合率.
相关概念视频
01:18Amino Acid Catabolism
996
Microorganisms rely on proteins as an essential carbon and energy source, particularly in environments with limited polysaccharides or lipids. However, proteins are too large to cross the plasma membrane unaided, necessitating enzymatic degradation. Microbes secrete extracellular proteases and peptidases that hydrolyze proteins into peptides, which can then be transported across the membrane. Once inside the cell, intracellular proteases degrade these peptides into free amino acids, which...
996
01:18ATP Synthase: Structure
15.1K
ATP synthase or ATPase is among the most conserved proteins found in bacteria, mammals, and plants. This enzyme can catalyze a forward reaction in response to the electrochemical gradient, producing ATP from ADP and inorganic phosphate. ATP synthase can also work in a reverse direction by hydrolyzing ATP and generating an electrochemical gradient. Different forms of ATP synthases have evolved special features to meet the specific demands of the cell. Based on their specific feature, ATP...
15.1K
01:48ATP Synthase: Mechanism
16.7K
In animals, the mitochondrial F1F0 ATP synthase is the key protein that synthesizes ATP molecules through a complex catalytic mechanism. While the nuclear genome encodes the majority of ATP synthase subunits, the mitochondrial genome encodes some of the enzyme's most critical components. The formation of this multi-subunit enzyme is a complex multi-step process regulated at the level of transcription, translation, and assembly. Defects in one or more of these steps can result in decreased...
16.7K
01:27ATP Driven Pumps I: An Overview
9.7K
ATP-driven pumps, also known as transport ATPases, are integral membrane proteins. They have binding sites for ATP located on the membrane's cytosolic side and the ion-conducting domain in the transmembrane region. These pumps use the free energy released from ATP hydrolysis to move the solutes across cell membranes against an electrochemical gradient.
There are four main types of ATP-driven pumps - P-type, V-type, F-type, and ABC transporter. All these pumps are of varying complexities and...
There are four main types of ATP-driven pumps - P-type, V-type, F-type, and ABC transporter. All these pumps are of varying complexities and...
9.7K
01:18Detergent Purification of Membrane Proteins
6.3K
Detergents are used to purify the integral proteins of the membrane. The hydrophobic portion of the detergent can replace membrane phospholipids while solubilizing the membrane proteins. When detergent monomers reach a specific concentration in a solution called critical micelle concentration (CMC), they form micelles. Above CMC, the concentration of the detergent monomers remains in equilibrium with the micelle. The number of detergent monomers present in the CMC varies for each detergent, and...
6.3K
01:19Allosteric Proteins-ATCase
6.5K
Binding sites linkages can regulate a protein's function. For example, enzyme activity is often regulated through a feedback mechanism where the end product of the biochemical process serves as an inhibitor.
Aspartate transcarbamoylase (ATCase) is a cytosolic enzyme that catalyzes the condensation of L-aspartate and carbamoyl phosphate to N-carbamoyl-L-aspartate. This reaction is the first step in pyrimidine biosynthesis. UTP and CTP, the end products of the pyrimidine synthesis...
Aspartate transcarbamoylase (ATCase) is a cytosolic enzyme that catalyzes the condensation of L-aspartate and carbamoyl phosphate to N-carbamoyl-L-aspartate. This reaction is the first step in pyrimidine biosynthesis. UTP and CTP, the end products of the pyrimidine synthesis...
6.5K