分析前因素和生物变异性对尿液挥发体的影响
 Carapito1,2, F Amaro3,4, A Teixeira-Marques5,6
1Associate Laboratory i4HB - Institute for Health and Bioeconomy, University of Porto, Porto, Portugal. up201804198@up.pt.
Analytical and bioanalytical chemistry
|October 15, 2025
概括
标准化预分析条件对于可靠的尿液挥发性有机化合物 (VOC) 生物标志物发现至关重要. 这项研究揭示了禁食,样品处理和储存如何影响VOC档案,强调了在代谢工作流程中需要严格的协议.
科学领域:
- 代谢学 代谢学 代谢学
- 分析化学 分析化学
- 生物标志物发现发现
背景情况:
- 尿液中的挥发性有机化合物 (VOC) 显示出作为非侵入性疾病生物标志物的潜力.
- 挥发性有机化合物的挥发性使它们易受分析前的变化,挑战可重现性.
- 在代谢学研究中,标准化对于一致的生物标志物发现至关重要.
研究的目的:
- 评估分析前变量对尿液VOC和挥发性碳烯化合物 (VCC) 概况的影响.
- 评估禁食,离心,温度稳定性和冷解周期的影响.
- 为了确定尿液VOC测量的个体内和个体间的变化.
主要方法:
- 用头部空间固相微提取与气色谱质谱学 (HS-SPME/GC-MS) 结合分析的尿样.
- 禁食,标准与超离心,室温 (RT) 稳定性 (高达21小时) 和冷解 (FT) 周期的研究影响.
- 评估了2.5个月的个人内和个人间变异性,采用了8个采集.
主要成果:
- 禁食状态显著改变了特定的VOC和VCC水平,尽管多变量分析显示没有差异.
- 在标准离心和超离心后,尿液中的VOC/VCC概况相似.
- 在RT时,VOC档案在21h之前保持稳定,而在14h之后,VOCs发生了变化.
- 重复的FT周期在两个周期后引起了显著的VOC变化;VCC保持稳定.
- 高的个人内和个人间变异性 (RSD>30%,ICC <0.4) 表明时间可靠性有限.
结论:
- 分析前的因素显著影响尿液中的VOC和VCC概况.
- 采样,处理和储存的标准化协议对于可重复性至关重要.
- 严格的标准化将提高VOCs作为代谢学中的生物标志物的可靠性.
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