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使用基因共同表达网络分析,识别与Histia rhodope幼虫过冬相关的关键基因

Haibo Yang¹, Shien Pang¹, Shanshan Guo¹

¹College of Horticulture and Plant Protection, Henan Provincial Engineering Technology Research Center of Green Plant Protection, Henan University of Science and Technology, Luoyang, China.

|October 15, 2025

概括

这项研究揭示了参与Histia rhodope幼虫过冬的关键基因,为寒冷耐受机制提供了洞察力. 了解这些分子通路可以帮助管理这种农业害虫.

科学领域:

昆虫学昆虫学是一门学科.
分子生物学分子生物学
生态生态学生态生态学

背景情况:

红草 (Histia rhodope) 是一种严重的害虫,会影响Bischofia polycarpa树,影响城市景观和经济.
冬季过冬的H. rhodope幼虫的策略,通常在子中,受到低温的影响,需要研究它们的分子耐寒性.

研究的目的:

探索在Histia rhodope幼虫的过冬过程背后的分子机制.
确定基因和通路对于这种昆虫害虫耐寒性至关重要.

主要方法:

用RNA测序 (RNA-seq) 来分析跨越五个过冬阶段的转录组.
权重基因共同表达网络分析 (WGCNA) 和蛋白质-蛋白质相互作用 (PPI) 网络分析被用来识别关键基因及其相互作用.
使用定量逆转录聚合酶链反应 (qRT-PCR) 来验证RNA-seq发现.

主要成果:

确定了30个与寒冷耐受性相关的基因,其中枢纽基因包括转位子相关蛋白,硫素,甘氨酸裂解系统H蛋白,核糖体蛋白和ATP合成酶.
还确定了细胞染色体P450基因.
核糖体蛋白和热冲击蛋白90在PPI网络中显示出高的相互作用潜力,这表明它们在过冬生存中的作用.

结论:

来自H. rhodope的五个过冬阶段的转录组数据揭示了与过冬过程相关的关键基因.
这项研究为未来对H. rhodope.冬季过冬的分子基础的研究提供了基础的理解.

关键词:

耐寒性耐寒性耐寒性耐寒性希斯蒂亚·罗多普 (Histia rhodope) 是一种植物.哈勃基因哈勃基因哈勃基因哈勃基因在冬季过冬.在RNA-seqqq.在WGCNA中,WGCNA是WGCNA.

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