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在天然鱼杂交物 (Fundulus diaphanus × F. heteroclitus) 中积累双亲遗传的海王星转移元素

Alexandre-James Roussel¹, Alexander Suh^2,3,4,5, Francisco J Ruiz-Ruano^2,3,4

¹Département de Biologie, Université de Moncton, 18 Antonine-Maillet avenue, Moncton, NB, Canada, E1A 3S4.

Molecular biology and evolution

|October 16, 2025

概括

由于piRNA沉默受损,可转移元素 (TE) 在Fundulus杂交物中积聚. 这表明混合体中的piRNA通路功能不足于最佳,导致活性TE积累和潜在的基因组不稳定性.

科学领域:

基因组学就是基因组学.
分子生物学分子生物学
进化生物学进化生物学

背景情况:

可转移元素 (TE) 是可引起基因组不稳定的移动遗传序列.
RNA干扰途径,特别是piwi相互作用RNA (piRNAs),对于抑制TE活性至关重要.
在跨物种杂交物种中,父亲遗传的TEs和TEs的减压表明潜在的piRNA通路功能障碍.

研究的目的:

在Fundulus heteroclitus和Fundulus diaphanus的自然杂交物中研究可转移元素 (TE) 负荷和活性.
确定非最佳的piRNA通路功能是否有助于这些杂交物中的TE积累.

主要方法:

比较基因组学以表征父母物种及其杂交物种的重复性DNA含量.
分析TE年龄和子家族活动,重点关注双亲遗传因素.
与混合繁殖模式相关的TE积累的评估.

主要成果:

混合动力表现出略高于预期的TE负载,年轻重复的丰富.
两个特定的海王星TE子家族在杂交品种中显示出显著的3至4倍积累.
观察到的TE积累模式与受损的piRNA介导沉默相一致.

结论:

在Fundulus杂交品种中,低于最佳的piRNA通路功能可能导致TE的活跃积累.
这种积累,特别是年轻和活跃的TE亚家族,可能会影响混合基因组的稳定性.
这些发现提供了自然跨物种杂交物种中TE介导的基因组应激的证据.

关键词:

无性繁殖是一种无性繁殖.鱼类鱼类鱼类鱼类的鱼类鱼类.混合化混合化混合化一个重复性的DNA DNA.可转移的元素可以转移.

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