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直接从血液培养样本中快速识别碳烯酶基因

Ghada A Ziad¹, Deena Jalal², Mohamed Hashem³

¹Microbiology Unit, Children's Cancer Hospital Egypt (CCHE-57357), Cairo 11441, Egypt.

Diagnostics (Basel, Switzerland)

|October 16, 2025

概括

该Xpert® Carba-R测定准确地检测直接从阳性血液培养的碳酶基因,使得更快的抗菌治疗碳酶产生肠杆菌 (CPE) 感染. 这种负担得起的方法为昂贵的系统提供了一种节省时间的替代方案.

科学领域:

临床微生物学临床微生物学
分子诊断学分子诊断学
抗微生物耐药性抗微生物耐药性

背景情况:

碳酶生成肠细菌 (CPE) 感染需要快速识别才能有效治疗.
目前用于CPE耐药基因的商业PCR系统非常昂贵.
该Xpert® Carba-R测定是一种敏感和特定的,但又负担得起的选择,用于检测碳烯酶基因.

研究的目的:

评估Xpert® Carba-R测试对从阳性血液培养物中直接检测碳酶基因的性能.
将试验结果与全基因组测序 (WGS) 作为黄金标准进行比较.
评估该试验在加快对CPE感染的抗微生物治疗中的有用性.

主要方法:

一项前性观察性研究,涉及590个阳性血液培养与格拉姆阴性棒.
使用Xpert® Carba-R测定方法对阳性血液培养瓶进行直接测试.
将Xpert® Carba-R结果与全基因组测序 (WGS) 数据进行比较.

主要成果:

在Xpert® Carba-R试验中,在590个标本中的178个中,在大约一个小时内,Xpert® Carba-R试验在590个标本中的178个中确定了碳酶基因.
流行的基因型包括blaNDM,blaOXA-48-like,以及双blaNDM/blaOXA-48-like.
在Xpert® Carba-R测定和WGS之间观察到近乎完美的一致性,用于基因型抵抗模式和个体基因检测.

结论:

Xpert® Carba-R测定是一种准确,负担得起和节省时间的工具,用于从血液培养物中直接检测碳烯酶基因.
这种方法有助于快速启动适合的CPE感染的抗菌疗法.
该测试为寻求高效的CPE诊断的临床实验室提供了实用解决方案.

关键词:

全基因组测序 (WGS) 是一种全基因组测序.在Xpert® Carba-R测定试验中,血液文化血液文化碳烯酶基因的基因诊断微生物学诊断微生物学

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