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牛KLF基因家族的全面识别及其在肌肉发育中的功能调节:从单核转录组学的见解

Fengying Ma^1,2, Le Zhou^1,2, Lili Guo³

¹College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China.

Animals : an open access journal from MDPI

|October 16, 2025

概括

这项研究描述了牛骨肌肉中的克鲁佩尔样因子 (KLF) 基因家族,确定了影响特定品种肌肉发育的关键KLF基因,并为改善肉类生产提供了目标.

科学领域:

基因组学就是基因组学.
分子生物学分子生物学
动物科学动物科学

背景情况:

克鲁佩尔样因子 (KLF) 家族调节重要的生物过程,如脂肪生成,肌形成和新陈代谢.
KLF基因家族的表征在人类和小鼠中广泛,但在牛中有限,特别是在骨肌肉中.
了解KLF在牛中的作用对于改善肉类生产特征至关重要.

研究的目的:

系统地描述KLF基因家族在Bos Taurus中的特征.
阐明KLF基因在牛特定品种肌肉发育中的作用.
通过分子育种来确定提升肉类生产的候选基因.

主要方法:

使用了比较基因组学和单核RNA测序 (snRNA-seq).
对Angus (牛肉) 和Holstein (奶牛) 品种牛的长腰背肌进行了分析.
机器学习算法被用来识别关键的转录驱动因素.

主要成果:

遗传学分析确定了14个KLF基因,显示了进化保护和潜在的功能分歧.
在11个不同的细胞群中,snRNA-seq揭示了KLF的细胞类型特定表达模式.
确定KLF6,KLF9,KLF10和KLF12是牛品种之间转录差异的主要驱动因素;KLF6在淋巴内皮细胞中显著.

结论:

这项研究为KLF在牛群中的家族研究提供了基础资源.
已识别的KLF基因是通过分子育种策略改善肉类生产特征的有希望的候选者.
这些发现有助于更深入地了解牛骨肌肉发育调节.

关键词:

这就是Bos Taurus.像克鲁佩尔这样的因素.这就是 SHAP SHAP 的意思.长极长的背部 (longissimus dorsi) 是一个很长的背部.单核RNA测序的一个核.

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