通过高速超离心法分离的等离子体衍生纳米颗粒的形态特征:扫描电子显微镜研究
Lubov A Kungurova1, Alexander A Artamonov1, Evgeniy A Grigoryev2
1S. M. Kirov Military Medical Academy, St. Petersburg 194044, Russia.
International journal of molecular sciences
|October 16, 2025
概括
研究人员使用低压扫描电子显微镜分析外聚体和超聚体,这是细胞通信中的关键纳米粒子. 这种方法可视化了人体血中的这些小颗粒 (10-50 nm),有助于了解它们的尺寸和结构.
科学领域:
- 纳米技术和材料科学 材料科学
- 细胞生物学和生物化学
- 生物物理学和成像技术
背景情况:
- 细胞外囊泡 (EVs) 是已建立的细胞间信号传递的媒介.
- 更小的,非囊状纳米颗粒,外聚体和超聚体,也在细胞通信中发挥作用.
- 描述这些纳米级实体对于理解它们的生物功能至关重要.
研究的目的:
- 为了研究从人体血中分离出来的外体和超体的大小.
- 评估低压扫描电子显微镜 (LVSEM) 对可视化这些纳米粒子的实用性.
- 描述生物流体中的纳米级细胞间通信粒子.
主要方法:
- 从血小板贫乏的人类血中通过顺序高速超离心法分离出外聚和超聚.
- 在未染色的Zeiss Auriga显微镜上使用低压扫描电子显微镜 (LVSEM) 进行可视化和尺寸分析.
- 使用动态光散射 (DLS) 进行补充尺寸评估.
主要成果:
- LVSEM揭示了10-50纳米大小范围的粒子,与异构体和超构体一致,在分离的等离子体中.
- 动态光散射证实了10-18纳米大小的粒子存在.
- LVSEM展示了对外体和超级体的可视化潜力,但注意到在将其与没有免疫标记的其他粒子区分以及在接近检测值的精确尺寸确定方面存在局限性.
结论:
- 低压扫描电子显微镜是一种可行的技术,用于检查纯化血分量的外聚体和超聚体.
- 该研究成功地可视化了归因于外构和超构的纳米级粒子 (10-50 nm).
- 需要进一步的进展,可能包括免疫学识别,以使用LVSEM对这些纳米粒子进行最终的表征和歧视.
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