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尿病原菌大肠杆菌生物膜:抗生素压力和与人类中性粒细胞的相互作用

Irina L Maslennikova^1,2, Irina V Nekrasova¹, Marjanca Starčič Erjavec^3,4

¹Institute of Ecology and Genetics of Microorganisms Ural Branch Russian Academy of Sciences-Perm Federal Research Center Ural Branch Russian Academy of Sciences, 614081 Perm, Russia.

International journal of molecular sciences

|October 16, 2025

概括

尿病原性大肠杆菌 (UPEC) 生物膜中的抗生素耐药性复杂化了尿路感染 (UTI) 的治疗. 低抑制性抗生素度可能会意外地增加UPEC生物膜的形成,并促进细菌耐药性.

科学领域:

微生物学微生物学
传染性疾病传染性疾病
细菌学细菌学是一门学科.

背景情况:

泌尿病原性大肠杆菌 (UPEC) 是尿路感染 (UTI) 的主要原因之一.
复发性尿路感染通常与UPEC形成生物膜的能力有关,有助于抗生素耐药性.
了解抗生素压力下的UPEC生物膜动态对于有效的尿路感染治疗至关重要.

研究的目的:

研究各种抗生素对UPEC生物膜形成的影响.
评估UPEC生物膜与人类中性粒细胞之间的相互作用.
在浮游生物和生物膜状态下确定UPEC的抗生素耐药性概况.

主要方法:

确定安培素,胺素,氨基,氨基,氨基,氨基和氨基的最小抑制度 (MIC),最小杀菌度 (MBC) 和生物膜清除度 (MBEC).
对UPEC生物膜生物质量和形态学上的西普洛素亚抑制度 (sub-MICs) 的评估.
对UPEC生物膜和超剂对人类中性粒细胞活力和功能的影响的评估.

主要成果:

所有测试的抗生素都显示出比MBC更高的MBEC,这表明生物膜的耐药性增加.
齐普罗夫洛克萨的子MIC刺激了生物膜的形成,并诱导了UPEC (丝状细胞) 的形态变化.
嵌入生物膜的UPEC显著降低了中性粒细胞通过亡的活力,而超剂的影响最小.

结论:

矛盾的是,抗生素压力可以增强UPEC生物膜的形成,这对尿路感染治疗构成挑战.
研究抗生素对生物膜形成的影响对于开发针对复发性尿路感染的策略至关重要.
UPEC生物膜对中性粒细胞功能构成重大威胁,有助于尿路感染的发病.

关键词:

抗生素抗生素是一种抗生素.灭症 (apoptosis) 是一种死亡的过程.生物膜的形成.这就是西普罗夫洛克萨辛 (Ciprofloxacin).聚态核中性粒细胞 (PMN) 是一种多态核中性粒细胞.在MIC前的MIC前的MIC.亚MIC是指MIC以下的公司.泌尿病原性大肠杆菌 (UPEC) 是一种

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