相关实验视频
Updated: Jan 15, 2026

07:00
In Ovo Intravascular Injection in Chicken Embryos
Published on: June 3, 2022
7.1K
在胚胎早期的性别确定过程中识别关键的差异性表达基因
Ruijia Liu1,2, Huanhuan Miao2, Bo Zhang1,2
1Frontiers Science Center for Molecular Design Breeding (MOE), China Agricultural University, Beijing 100193, China.
International journal of molecular sciences
|October 16, 2025
概括
了解的性别决定是提高家禽养殖效率的关键. 这项研究确定NR2F1和NFIB是DMRT1的潜在激活剂,对胚胎早期的男性性发育至关重要.
科学领域:
- * 基因组学和发育生物学
- * 家禽科学与动物繁殖
背景情况:
- *准确的性别测定对于优化生产效率和解决密集家禽养殖的伦理问题至关重要.
- * 了解胚胎性腺体分化背后的分子机制对于推进这种经济重要物种的繁殖策略至关重要.
研究的目的:
- * 为了在胚性性腺体中识别差异表达基因 (DEGs),在化后3.5天.
- *阐明关键基因和通路在早期性别确定和性腺分化中的潜在作用.
- * 调查参与DMRT1表达的上游调节因素,这是一个关键的男性性别决定基因.
主要方法:
- *从3.5天老的白色莱戈恩胚胎 (15个雄性,15个雌性) 的生殖器脊的转录组测序.
- *生物信息分析以确定性别之间显著差异表达的基因 (DEGs).
- *使用定量实时PCR (RT-qPCR) 验证关键的DEG.
- *生物信息学预测基因促进体内转录因子结合部位.
主要成果:
- *确定了283个在雄性和雌性胚胎生殖腺之间显著差异表达的基因 (DEGs).
- * 丰富分析显示,DEG参与了诸如核糖体生物发生,甘氨酸生物合成和TGF-β信号传递等途径.
- * 已验证的关键DEG,包括SMAD2Z,FREM1,NR2F1,SEMA6A,NFIB,RNF165,SMAD7B,SMAD2W,SPIN1W和HINTW. * 已验证的关键DEG,包括SMAD2Z,FREM1,NR2F1,SEMA6A,NFIB,RNF165,SMAD7B,SMAD2W,SPIN1W和HINTW. * 已验证的关键DEG,包括SMAD2Z,FREM1,NR2F1,SEMA6A,NFIB,RNF165,SMAD7B,SMAD2W,SPIN1W和HINTW.
- *在DMRT1促进区内预测NR2F1和NFIB的结合点,表明它们作为上游激活剂的作用.
结论:
- *NR2F1和NFIB被确定为早期雄胚胎中DMRT1表达的潜在上游激活剂.
- *这些发现有助于了解家禽的性别决定和丸发育的遗传调节.
- *这项研究提供了对胚胎性腺区分至关重要的分子通路的见解,有助于未来的家禽繁殖进步.
相关概念视频
The Ratio of X Chromosome to Autosomes
9.4K
In most organisms, sex is determined by the ratio of X and Y chromosomes. However, in some organisms, such as Drosophila and C.elegans, sex is determined by the ratio of the number of X chromosomes to the number of sets of autosomes. The Y chromosome in Drosophila is active but does not determine sex. It contains genes responsible for the production of sperms in adult flies.
Normal male Drosophila has a ratio of one X chromosome to two sets of autosomes. In contrast, normal female...
Normal male Drosophila has a ratio of one X chromosome to two sets of autosomes. In contrast, normal female...
9.4K
Development of the Sexual Organs in the Embryo and Fetus
3.3K
Development of the reproductive organs in an embryo starts from a bipotential state. This means the early embryo can develop either male or female reproductive organs. The formation of these organs begins with the growth of gonadal ridges that arise from the intermediate mesoderm during the fifth week of development.
Near the gonadal ridges, two duct systems are present: the mesonephric ducts (Wolffian ducts) and paramesonephric ducts (Müllerian ducts). These ducts form the basis for the...
Near the gonadal ridges, two duct systems are present: the mesonephric ducts (Wolffian ducts) and paramesonephric ducts (Müllerian ducts). These ducts form the basis for the...
3.3K
Determination
20.8K
During embryogenesis, cells become progressively committed to different fates through a two-step process: specification followed by determination. Specification is demonstrated by removing a segment of an early embryo, “neutrally” culturing the tissue in vitro—for example, in a petri dish with simple medium—and then observing the derivatives. If the cultured region gives rise to cell types that it would normally generate in the embryo, this means that it is specified. In...
20.8K
Gastrulation
66.2K
Gastrulation establishes the three primary tissues of an embryo: the ectoderm, mesoderm, and endoderm. This developmental process relies on a series of intricate cellular movements, which in humans transforms a flat, “bilaminar disc” composed of two cell sheets into a three-tiered structure. In the resulting embryo, the endoderm serves as the bottom layer, and stacked directly above it is the intermediate mesoderm, and then the uppermost ectoderm. Respectively, these tissue strata...
66.2K

