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相关概念视频

Proteoglycans01:05

Proteoglycans

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Glycans, a class of complex heterogeneous molecules, can be covalently attached to proteins to form glycosylated proteins that regulate various physiological and pathological processes. Glycosylated proteins or glycoproteins comprise N-linked and O-linked oligosaccharides. O-glycosylation is the most common type of protein glycosylation. Here, glycans attach to the oxygen atom of the hydroxyl groups of Serine or Threonine residues. O-linked glycosylation occurs later in protein processing,...
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Glycosaminoglycans01:23

Glycosaminoglycans

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Glycosaminoglycans (GAGs), also known as mucopolysaccharides, are long and linear polymers comprising of specific repeating disaccharides - the amino sugar that can be N-acetylglucosamine or N-acetylgalactosamine, and a uronic acid that is usually glucuronic acid or iduronic acid.
GAGS are found in the extracellular matrix of vertebrates, invertebrates, and bacteria. Due to their polar nature they attract water, and serve as excellent lubricants or shock absorbers in an animal body.
Hyaluronic...
6.8K
Oligosaccharide Assembly01:24

Oligosaccharide Assembly

3.5K
Protein glycosylation starts in the ER lumen and continues in the Golgi apparatus. Glycosyltransferases catalyze the addition of sugar molecules or glycosylation of proteins. Usually, these enzymes add sugars to the hydroxyl groups of selected serine or threonine residues to form O-linked glycans or the amino groups of asparagine residues to form N-linked glycans. Different positions on the same polypeptide chain can contain differently linked glycans.
Multiple sugar molecules that may or may...
3.5K
Protein Glycosylation01:25

Protein Glycosylation

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Glycosylation, the most common post-translational modification for proteins, serves diverse functions. Adding sugars to proteins makes the proteins more resistant to proteolytic digestion. Glycosylated proteins can act as markers and receptors to promote cell-cell adhesion. Additionally, they have many essential quality control functions in the cell, such as correct protein folding and facilitating transport of misfolded proteins to the cytosol, which can be degraded.
Glycosylation occurs in...
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T Cell Types and Functions01:24

T Cell Types and Functions

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When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
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Rh Blood Group01:19

Rh Blood Group

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The Rhesus (Rh) antigen is crucial in determining blood groups and ensuring compatibility during blood transfusions.
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相关实验视频

Updated: Jan 15, 2026

Adoptive Immunotherapy of iNKT Cells in Glucose-6-Phosphate Isomerase G6PI-Induced RA Mice
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Adoptive Immunotherapy of iNKT Cells in Glucose-6-Phosphate Isomerase G6PI-Induced RA Mice

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在风湿性关节炎中免疫球蛋白G子类特定的糖化变化.

Dániel Szabó1,2, Balázs Gyebrovszki3, Eszter Szarka3

  • 1MS Proteomics Research Group, HUN-REN Research Centre for Natural Sciences, 1117 Budapest, Hungary.

International journal of molecular sciences
|October 16, 2025
PubMed
概括

这项研究表明,在类风湿性关节炎 (RA) 中,抗素蛋白抗体 (ACPA) 的N-糖化与疾病严重程度相关. 异形特异性分析对于理解IgG糖化中的这些复杂变化至关重要.

关键词:
抗素蛋白抗体的抗素蛋白抗体.关节炎是一种关节炎.葡萄糖酶化是什么? 葡萄糖酶化是什么?免疫球蛋白G异型蛋白的异型蛋白.类风湿症患者的情况

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Immunoglobulin G N-Glycan Analysis by Ultra-Performance Liquid Chromatography
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Immunoglobulin G N-Glycan Analysis by Ultra-Performance Liquid Chromatography
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科学领域:

  • 免疫学 免疫学 免疫学
  • 葡萄糖生物学 葡萄糖生物学
  • 类风湿病学 类风湿病学

背景情况:

  • 风湿性关节炎 (RA) 是一种常见的炎症性多关节炎.
  • 抗素蛋白抗体 (ACPA) 是关键的诊断标志物,在60-80%的患者中存在.
  • 对IgG自身抗体的FcN-糖化显著影响了它们的效应器功能.

研究的目的:

  • 在相同的RA患者和健康对照中,将ACPA IgG的Fc N-糖化与非ACPA IgG进行比较.
  • 在RA中研究IgG异形特异性糖基化模式.
  • 为了将糖基化特征与临床炎症标志物和疾病活性评分相关联.

主要方法:

  • 从患者和对照组中分离ACPA和正常血清IgG.
  • 素消化IgG和混合物分离使用逆相纳米LC.
  • 使用布鲁克马克西斯II Q-TOF质谱仪对糖形丰度的分析.

主要成果:

  • 发现了ACPA IgG的银河系化/化与炎症标志物 (CRP,ESR) 和类风湿因子 (RF) 之间显著的负相关性.
  • 非ACPA IgG 显示了糖化和疾病活性评分 (DAS) 之间的显著负相关性.
  • 对同位体特异性分析显示,IgG1解释了分割GlcNAc的变化,而fucosylation不变的结果来自两个同位体的对立变化.

结论:

  • 在RA患者中,IgG的FcN-糖化模式,特别是以异型特异的方式发生变化.
  • 对ACPA IgG和非ACPA IgG的糖化表明与疾病标志物有明显的相关性.
  • 异形特异性分析对于全面了解RA发病的IgG糖化和作为潜在的生物标志物至关重要.