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在氧化应激下,Yarrowia lipolytica细胞的抗氧化系统反应

Gerardo Ismael Arredondo-Mendoza¹, Maripaz Castillo-Roque¹, Hipólito Otoniel Miranda-Roblero¹

¹Universidad Autónoma de Nuevo León, Centro de Investigación en Nutrición y Salud Pública, Facultad de Salud Pública y Nutrición, Av. Dr. Eduardo Aguirre Pequeño y Yuriria, Col. Mitras Centro, Monterrey CP. 64460, Nuevo León, Mexico.

International journal of molecular sciences

|October 16, 2025

概括

在应对氧化应激 (OS) 时,Yarrowia lipolytica通过增加关键酶如催化酶和超氧化脱酶的基因表达来激活其抗氧化防御系统 (ADS). 年轻细胞对OS的转录反应比老细胞更强.

科学领域:

微生物学微生物学
分子生物学分子生物学
生物化学生物化学

背景情况:

细胞拥有抗氧化防御系统 (ADS),以抵消由活性氧物种 (ROS) 引起的氧化应激 (OS).
虽然在许多物种中已知ADS机制,但Yarrowia lipolytica对OS的特定反应仍未得到充分研究.
ADS激活涉及翻译后修改和基因表达诱导,以保护细胞组件免受ROS损伤.

研究的目的:

为了研究Yarrowia lipolytica*中氧化应激反应背后的分子机制.
分析抗氧化酶基因的转录调节,包括超氧化脱酶 (SOD),酶 (CAT) 和谷氨过氧化酶 (GPX),在Y. lipolytica中.
为了在氧化应激下比较年轻和老年Y. lipolytica*细胞之间的ADS基因表达特征.

主要方法:

在暴露于过氧化 (H2O2) 时评估Y. lipolytica* 基因 (*CAT*, *SOD*, *CCS*, *GPX*) 的表达特征.
在H2O2治疗后,随着时间的推移量化基因表达水平.
在OS条件下对年轻与老年Y. lipolytica*细胞的基因表达进行比较分析.

主要成果:

*Y. lipolytica* 拥有三个 *CAT* 基因,一个 *SOD* 基因,一个 *CCS* 基因和一个 *GPX* 基因.
所有分析的抗氧化基因的表达在H2O2暴露后15分钟内达到峰值.
与老年细胞相比,年轻的Y. lipolytica细胞对所有ADS基因的表达水平显著更高,其中CAT3和SOD显示出最明显的上调.

结论:

*Yarrowia lipolytica*中的抗氧化防御系统在转录水平上受到调节.
由*CAT3*和*SOD*编码的酶在Y. lipolytica*的氧化应激反应中起着至关重要的作用.
细胞年龄会影响Y. lipolytica对氧化应激的转录反应,年轻的细胞会建立更强大的防御能力.

关键词:

雅罗维亚 (Yarrowia lipolytica) 是一个高脂的植物.抗氧化剂反应的抗氧化剂反应基因表达的基因表达方式氧化应激是一种氧化应激.

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