细菌细菌DinG 3'5'外核酶:一个帮助减轻复制应激的助手
Begoña Carrasco1, Rubén Torres1, María López-Sanz1
1Department of Microbial Biotechnology, Centro Nacional de Biotecnología (CNB-CSIC), Calle Darwin 3, 28049 Madrid, Spain.
International journal of molecular sciences
|October 16, 2025
概括
细菌细菌 DinG 蛋白质降解单链 DNA 和 RNA,去除 R 环,并帮助转化 DNA 合成 (TLS),以减轻复制压力.
科学领域:
- 分子生物学分子生物学
- DNA 修复机制的修复机制
- 酶学 是一种酶学.
背景情况:
- 细菌细菌DinG和YpvA是参与管理复制应激的XPD类类型对应物.
- DinG 含有 DEDD 外核酶和 DNA 螺旋酶域,而 YpvA 缺乏外核酶域.
研究的目的:
- 描述Bacillus subtilis DinG.的酶活性和生物功能.
- 研究DinG在DNA修复途径中的作用,特别是在应对复制压力的过程中.
主要方法:
- 生物化学分析以确定DNA/RNA降解和结合亲和力.
- 分析DinG与DNA结构的相互作用,例如分叉DNA和RNA-DNA杂交.
- 使用遗传方法和显微镜研究DinG在突变发生中的作用及其与其他蛋白质 (RecA,PolA) 的物理相互作用.
主要成果:
- DinG在线性单链DNA上表现出3'→5'外核酶活性,并在RNA-DNA杂交体内降解RNA.
- DinG 破坏稳定并降解分叉的 DNA 结构,而 ATP 在双重结之前促进解离.
- DinG与ReCA和PolA相互作用,在转化合成 (TLS) 中起作用,并在复制分叉中形成焦点,特别是在DNA损伤诱导后.
结论:
- DinG通过降低R循环结构和促进TLS来减轻复制压力,可能是通过ReCA介导的途径.
- 通过解决DNA复制障碍,DinG在维持基因组稳定性方面发挥着至关重要的作用.
更多相关视频
06:25Quantifying Replication Stress in Ovarian Cancer Cells Using Single-Stranded DNA Immunofluorescence
Published on: February 10, 2023
2.4K
09:04Studying Ribonucleotide Incorporation: Strand-specific Detection of Ribonucleotides in the Yeast Genome and Measuring Ribonucleotide-induced Mutagenesis
Published on: July 26, 2018
8.2K
相关概念视频
02:10Translesion DNA Polymerases
11.0K
Translesion (TLS) polymerases rescue stalled DNA polymerases at sites of damaged bases by replacing the replicative polymerase and installing a nucleotide across the damaged site. Doing so, TLS allows additional time for the cell to repair the damage before resuming regular DNA replication.
TLS polymerases are found in all three domains of life - archaea, bacteria, and eukaryotes. Of the different classes of TLS polymerases, members of the Y family are fitted with specialized structures that...
TLS polymerases are found in all three domains of life - archaea, bacteria, and eukaryotes. Of the different classes of TLS polymerases, members of the Y family are fitted with specialized structures that...
11.0K
01:20Mismatch Repair
6.3K
Organisms are capable of detecting and fixing nucleotide mismatches that occur during DNA replication. This sophisticated process requires identifying the new strand and replacing the erroneous bases with correct nucleotides. Mismatch repair is coordinated by many proteins in both prokaryotes and eukaryotes.
The Mutator Protein Family Plays a Key Role in DNA Mismatch Repair
The human genome has more than 3 billion base pairs of DNA per cell. Prior to cell division, that vast amount of genetic...
The Mutator Protein Family Plays a Key Role in DNA Mismatch Repair
The human genome has more than 3 billion base pairs of DNA per cell. Prior to cell division, that vast amount of genetic...
6.3K
01:36Mismatch Repair
43.5K
Overview
43.5K
01:02The DNA Replication Fork
40.4K
An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork. Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication...
40.4K
01:02The DNA Replication Fork
18.1K
18.1K
02:31Homologous Recombination
62.6K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
62.6K