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工程CO₂-将碳素酶体固定在Saccharomyces cerevisiae中,以改善乙醇生产

Mengqi Li¹, Simin Zeng¹, Yunling Guo¹

¹National Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan 430070, China.

International journal of molecular sciences

|October 16, 2025

概括

研究人员通过重建称为 carboxysomes 的细菌微分区来设计酵母来产生更多的乙醇. 这提高了代谢效率,增加了乙醇产量并减少了甘油副产品.

科学领域:

生物技术是生物技术.
合成生物学合成生物学
代谢工程是代谢工程.

背景情况:

细菌微分区 (BMCs) 分区细菌中的代谢途径.
碳氧体,一种BMC,使用蛋白质外来封装用于二氧化碳 (CO2) 固定的酶.
有效的二氧化碳固定对于各种生物技术应用至关重要.

研究的目的:

在酵母菌中重建一个功能性α-碳糖体.
为了研究将α-carboxysomes引入酵母的代谢影响.
评估使用工程酵母增强生物乙醇生产的潜力.

主要方法:

来自cso操作子 (Halothiobacillus neapolitanus),基化酶 (PRK) (Spinacia oleracea) 和陪伴基因 (HSP60,HSP10) 的基因被引入到S. 类植物. 类植物.
评估了核糖-1,5-双酸碳糖酶/氧化酶 (Rubisco) 的组合和活性.
使用糖密度梯度离心法来净化和确认α-碳素体结构.

主要成果:

工程酵母菌株成功组装并证明了Rubisco在α-carboxysome中的酶活性.
观察到乙醇生产的显著增加.
在副产品甘中实现了显著的减少.

结论:

在Saccharomyces cerevisiae中,可以成功地重建功能性α-carboxysomes.
工程酵母表现出增强的代谢效率,从而改善了生物乙醇生产.
这项工作为优化工业发酵过程提供了一个有前途的战略.

关键词:

哈洛西奥巴西勒斯尼阿波利塔努斯 (Halothiobacillus neapolitanus) 是一种植物.这种植物是Saccharomyces cerevisiae.细菌微分区是细菌的微分区.生物乙醇生产生物乙醇生产二氧化碳的固定方式的α-碳素酶组.

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