臭氧盐溶液将微质细胞两极化,使其转向一种抗炎现象型
Federica Armeli1, Beatrice Mengoni1, Martina Menin1
1Department of Medico-Surgical Sciences and Biotechnologies, Sapienza University of Rome, 04100 Latina, Italy.
Molecules (Basel, Switzerland)
|October 16, 2025
概括
低剂量臭氧化盐溶液 (臭氧疗法) 增强细胞生长和抗氧化防御,同时减少免疫细胞中的炎症. 这表明了安全的全身臭氧治疗的潜力.
科学领域:
- 生物医学科学 生物医学科学
- 免疫学 免疫学 免疫学
- 细胞生物学 细胞生物学
背景情况:
- 臭氧 (O3) 治疗显示出抗氧化和抗炎作用.
- 臭氧化盐溶液 (O3SS) 的全身用药尚未得到充分研究.
研究的目的:
- 评估O3SS对微质细胞 (BV2) 和内皮细胞 (HUVEC) 的细胞毒性,抗氧化和免疫调节作用.
- 探索O3SS作为全身臭氧治疗的潜力.
主要方法:
- 细胞接受了不同剂量的O3SS (1,5,10μg/NmL) 的治疗.
- 细胞毒性使用可行性试验进行评估.
- 测量了抗氧化剂基因表达 (Nrf2,SOD1) 和活性氧物种 (ROS) 的水平.
- 微细胞表型通过量化促炎 (iNOS,IL-1β) 和抗炎 (Arg-1,IL-10) 标志物进行了分析.
主要成果:
- 低剂量的O3SS (1.5μg/NmL) 促进了细胞增殖,没有毒性.
- 高剂量的O3SS (10μg/NmL) 降低了细胞活力,增加了细胞死亡.
- 在LPS刺激的微质中,O3SS提高了抗氧化基因的调节,并降低了ROS.
- O3SS将微质表型转移到抗炎状态,由增加的Arg-1表达证实.
结论:
- 低剂量的O3SS激活细胞抗氧化防御,并促进抗炎微质表型.
- O3SS显示出作为一种安全的全身臭氧治疗的潜力.
- 需要进一步的体内研究来确认疗效并优化临床方案.
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