构建和比较UPLC-QE-Orbitrap-MS和UPLC-MS/MS方法用于检测复杂水域矩阵中的亚兹帕姆残留物
Yuanhao Yang1,2,3, Yi Li2,3, Qiangbing Tian2,3
1College of Food Science and Technology, Northwest University, Xi'an 710069, China.
Foods (Basel, Switzerland)
|October 16, 2025
概括
一种新方法使用液态染色体质谱学准确量化水产产品中的亚兹帕姆及其代谢物. 这项经过验证的协议确保了食品安全和环境监测的可靠微量分析.
科学领域:
- 分析化学 分析化学
- 环境科学 环境科学
- 法医毒理学 法医毒理学
背景情况:
- 迪亚泽巴姆及其代谢物是常见的制药污染物.
- 在水生产品等复杂矩阵中精确检测具有挑战性.
- 现有的方法可能缺乏微量级分析的灵敏度或特异性.
研究的目的:
- 开发和验证标准化预治疗方案,用于同时测定亚泽巴姆及其代谢物 (诺达泽巴姆,氧泽巴姆,特马泽巴姆).
- 建立一个可靠的分析方法来量化水产品中的这些化合物.
- 为此分析,比较两个液态染色体质谱平台的性能.
主要方法:
- 样品采用0.2%的甲酸在乙甲中提取,并通过MCX固相提取净化.
- 两个液态染色体质谱平台 (UPLC-QE-Orbitrap-MS和UPLC-MS/MS) 进行了优化.
- 方法验证遵循食品法典的指导方针,评估线性,检测/定量限制,可重现性和回收.
主要成果:
- 取得了优异的线性 (R2 > 0.997) 和低检测极限 (0.1μg/kg) 和量化极限 (0.2μg/kg).
- 展示了高的跨平台一致性 (Pearson r > 0.990) 和良好的可重现性 (RSDs < 15%).
- 恢复率从73.8%到117.9%不等,这表明方法的准确性.
结论:
- 一种经过验证的双平台液态染色体质谱法方法使得在水产产品中准确地量化了泽及其代谢物的痕量.
- 开发的协议提供了高灵敏度,矩阵干扰耐受性和出色的可重现性.
- 这种方法适用于日常监测和确保水生食物来源的安全.
更多相关视频
05:46Identification of Pharmaceuticals in The Aquatic Environment Using HPLC-ESI-Q-TOF-MS and Elimination of Erythromycin Through Photo-Induced Degradation
Published on: August 1, 2018
13.5K
10:13Author Spotlight: An Efficient Methodology to Confidently Differentiate and Characterize Fentanyl Analogs
Published on: November 8, 2024
2.8K
相关概念视频
01:15High-Performance Liquid Chromatography: Types of Detectors
1.5K
The role of the detectors in High-Performance Liquid Chromatography (HPLC) is to analyze the solutes as they exit from the chromatographic column. The detector recognizes the solute's property and generates corresponding electrical signals, which are converted into a readable graph of the detector's response versus elution time called a chromatogram at the computer. There are several types of HPLC detectors, each with its own advantages and limitations, depending on the analyte...
1.5K
01:21Mass Spectrometry: Complex Analysis
1.6K
Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
1.6K