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植物对酶相关免疫吸收试验的矩阵干扰,用于检测帕拉残留物

Linglong Chen^1,2, Ge Chen¹, Xing Zhang¹

¹Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, State Key Laboratory of Vegetable Biobreeding, Key Laboratory of Vegetable Quality and Safety Control, Ministry of Agriculture and Rural Affairs of China, Ministry of Agriculture Vegetable Product Quality Safety Risk Assessment Laboratory, Beijing 100081, China.

Foods (Basel, Switzerland)

|October 16, 2025

概括

植物基质显著干扰了用于检测帕拉残留的酶相关免疫吸收试验 (ELISA) 的准确性. 酸处理有效地减少了这种干扰,提高了测试可靠性.

科学领域:

分析化学分析化学
食品安全食品安全
生物化学生物化学

背景情况:

与酶相关的免疫吸收试验 (ELISA) 对于检测农药残留物,如parathion,至关重要.
复杂的蔬菜矩阵对ELISA的准确性构成重大挑战,限制了其应用.
了解矩阵干扰机制对于改善分析方法至关重要.

研究的目的:

为了研究植物矩阵组件对帕拉西昂残留物的干扰机制,ELISA.
为了验证酸处理在减轻植物基质干扰方面的有效性.
为了提高ELISA在蔬菜样本中检测帕拉的可靠性.

主要方法:

在ELISA上研究了叶绿素,白蛋白,葡萄糖,果糖和糖的干扰.
比较矩阵干扰指数 (I_m) 和酸处理前后的蔬菜样本的恢复率.
使用抗体-IgG-HRP结合作为干扰的关键指标.

主要成果:

植物矩阵添加显著干扰了ELISA,主要影响抗体-IgG-HRP结合.
酸治疗使矩阵干扰指数 (I_m) 从16-26%降低到10-13%.
用酸处理的样本显示了令人满意的尖端恢复率 (80-113%),表明干扰最小化.

结论:

植物基因组件在帕拉西残留分析中大大干扰ELISA的性能.
酸处理是减少矩阵干扰和提高ELISA准确性的有效方法.
这项研究为可靠检测农药残留物的减轻矩阵效应提供了关键的见解.

关键词:

免疫测试是一种免疫测试.矩阵干扰干扰是什么帕拉西翁残留物帕拉西翁残留物快速检测快速检测迅速检测蔬菜蔬菜是一种蔬菜.

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