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宫癌中存在的介质干细胞和瘤细胞之间的相互作用会影响巨细胞的极化

Eduardo Bautista-Sebastián^1,2, Víctor Adrián Cortés-Morales³, Guadalupe Rosario Fajardo-Orduña¹

¹Mesenchymal Stem Cell Laboratory, Oncology Research Unit, Oncology Hospital, National Medical Center (IMSS), Mexico City 06720, Mexico.

|October 16, 2025

概括

来自瘤的宫癌细胞和瘤中介细胞 (MSCs) 促进M2巨细胞的两极分化,增强免疫抑制和潜在的瘤生长. 正常的宫MSCs在巨细胞中没有诱导这种M2表型.

科学领域:

免疫学免疫学免疫学
在瘤学瘤学.
细胞生物学细胞生物学

背景情况:

巨细胞,特别是M2表型,在瘤发育中至关重要.
宫癌中介细胞 (MSCs) 调节T淋巴细胞活动.
CeCa-MSCs对瘤细胞诱导的巨细胞极化的影响以前没有被评估.

研究的目的:

为了研究正常的子宫 (NCx) 和CeCa-MSCs如何影响极化巨细胞中的子宫瘤细胞 (TCs).
分析暴露于TC和不同MSC的巨细胞的免疫调节和免疫抑制潜力.

主要方法:

共同培养系统涉及宫瘤细胞 (TC) 和正常宫MSC (NCx-MSC) 或宫癌MSC (CeCa-MSC).
对巨细胞表型标记物的分析 (例如CD163).
测量细胞内细胞因子的产生 (IDO,IL-4,IL-10,IL-6) 和T淋巴细胞的增殖.

主要成果:

与TCs/NCx-MSCs不同的是,TCs/CeCa-MSCs共同培养了偏向于M2表型的巨细胞,与TCs/NCx-MSCs不同.
在TCs/CeCa-MSC共同培养中的巨细胞显示IDO,IL-4和IL-10的产量增加,免疫抑制潜力增强.
TCs/NCx-MSCs共同培养导致巨细胞的细胞能力降低和调控性T细胞生成,IL-6分泌量增加.

结论:

与CeCa-MSC相互作用的宫瘤细胞有效地将巨细胞两极分化为免疫抑制M2表型.
这种两极分化是由TC和CeCa-MSC之间的细胞内通信介导的,可能会导致宫癌的瘤生长.
这些发现突出了与瘤相关的MSCs促进免疫抑制瘤微环境的机制.

关键词:

的T淋巴细胞.宫癌:子宫癌是一种癌症.免疫调节免疫调节免疫调节巨细胞的两极分化来自瘤的介质细胞 stromal 细胞

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