相关实验视频

Updated: Jan 15, 2026

Characterization of Neuronal Lysosome Interactome with Proximity Labeling Proteomics

Characterization of Neuronal Lysosome Interactome with Proximity Labeling Proteomics

Published on: June 23, 2022

蛋白质组学对溶酶体生物发生和成熟的洞察

Katharina Hirn¹, Sofía Fajardo-Callejón^1,2, Dominic Winter^1,2

¹Department Metabolism, Senescence and Autophagy, Research Center One Health Ruhr, Medical Faculty, University Alliance Ruhr, University Hospital Essen, University of Duisburg-Essen, Essen, Germany.

|October 16, 2025

概括

溶解体是细胞回收的关键中心. 在过去的20年里,质谱蛋白质学显著提升了我们对溶酶体生物发生和功能的理解,识别了350多种关键蛋白质.

科学领域:

细胞生物学细胞生物学
生物化学生物化学
蛋白质组学是指蛋白质组学.

背景情况:

溶酶体是真核细胞中重要的降解器官,对于细胞平衡和代谢信号传递至关重要.
lysosomal 功能障碍与各种人类疾病有关,这强调了它们的重要性.
lysosomal生物发生是一个复杂的,多步骤的过程,需要精确控制酶活性和膜组装.

研究的目的:

在过去的二十年中,审查基于质谱 (MS) 的蛋白质组学对 lysosomal biogenesis 的特征的影响.
突出蛋白质组分析在理解 lysosome 功能和蛋白质识别中的作用.

主要方法:

lysosomes 和 lysosomal 蛋白质的丰富和蛋白质组分析.
基于质谱 (MS) 的蛋白质组学技术.

主要成果:

蛋白质组学在研究溶解体方面发挥了重要作用,包括基因表达控制,分类和贩运.
在过去的20年中,基于多发性硬化症的蛋白质组学已经确定了大约350种局部或关键的蛋白质.
这有助于更深入地了解控制 lysosomal biogenesis 的分子机制.

结论:

质谱蛋白质组学是一种强大的工具,用于剖析复杂的细胞过程,如 lysosomal biogenesis.
持续的蛋白质组研究将进一步阐明 lysosome生物学及其在健康和疾病中的作用.

关键词:

生物发生的生物发生.lysosomal 蛋白质是一种溶解体蛋白质.lysosome 溶解酶体是如何形成的质谱测量质谱测量质谱测量质谱测量质量测量质谱测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量成熟期成熟期成熟期贩卖蛋白质的人口贩运蛋白质组学蛋白质组学囊泡运输是一种囊泡运输.

更多相关视频

Study of Phagolysosome Biogenesis in Live Macrophages

Study of Phagolysosome Biogenesis in Live Macrophages

Published on: March 10, 2014

Evaluation of LC3-II Release via Extracellular Vesicles in Relation to the Accumulation of Intracellular LC3-positive Vesicles

Evaluation of LC3-II Release via Extracellular Vesicles in Relation to the Accumulation of Intracellular LC3-positive Vesicles

Published on: October 18, 2024

相关概念视频

Lysosomes

Lysosomes

Lysosomes are membrane-enclosed spherical sacs derived from the Golgi apparatus. The most important function of the lysosome is degrading macromolecules and biological polymers that are released during membrane trafficking events such as the secretory, endocytic, autophagic, and phagocytic pathways. The degradation is carried out by several hydrolytic enzymes active in an acidic environment of the lysosomal lumen. These acid hydrolases are involved in cellular processes such as cell signaling,...

Lysosomal Hydrolases

Lysosomal Hydrolases

Lysosomes are the site for the degradation of macromolecules and biological polymers released during membrane trafficking events such as secretory, endocytic, autophagic, and phagocytic pathways. The membrane-enclosed area of the lysosome, called the lumen, contains hydrolytic enzymes active in an acidic environment. These acid hydrolases are functional at a pH between 4.5 and 5 and are involved in cellular processes such as cell signaling, energy metabolism, restoration of the plasma membrane,...

Delivery Pathways to the Lysosome

Delivery Pathways to the Lysosome

Eukaryotic cells use different mechanisms to eliminate toxic waste obsolete and worn-out substances. Lysosomes play a pivotal role in this, and hence, these substances are carried to the lysosome from other parts of the cell and extracellular space through different pathways. The most elaborately studied pathways to the lysosome are the endocytic pathways.
Endocytosis
In endocytosis, the cell membrane takes up macromolecules and particles from the surrounding medium. Clathrin-mediated...

Maturation of Endosomes

Maturation of Endosomes

The early endosome containing internalized molecules matures through transformations in its location, morphology, intraluminal pH, and membrane protein composition. Together, these changes result in a more acidic late endosome that contains multiple intraluminal vesicles; therefore, the late endosome is also called a multivesicular body (MVB).
Changes in location
The maturing endosome moves along microtubules from the periphery of the cell towards the perinuclear region. This movement of the...

The Proteasome Structure

The Proteasome Structure

The ubiquitin-proteasome pathway is a well-known mechanism utilized by eukaryotic cells to remove cytoplasmic proteins that are misfolded, damaged, or no longer needed. In this pathway, the protein that needs to be eliminated undergoes a process called ubiquitination, where a chain of ubiquitin molecules is attached to the 48th lysine residue of the target protein. This ubiquitin modification helps the proteasome distinguish between a target protein and a healthy protein.
The proteasome is an...

The Proteasome

The Proteasome

Eukaryotic cells can degrade proteins through several pathways. One of the most important among these is the ubiquitin-proteasome pathway. It helps the cell eliminate the misfolded, damaged, or unwarranted cytoplasmic proteins in a highly specific manner.
In this pathway, the target proteins are first tagged with small proteins called ubiquitin. This involves participation of a series of enzymes including— E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme), and E3...