转录凝聚物和核孔综合体调节基因表达和3D基因组结构,以应对压力
Suman Mohajan1, David S Gross1
1Department of Biochemistry and Molecular Biology, Louisiana State University Health Sciences Center, Shreveport, LA 71130, U.S.A.
Biochemical Society transactions
|October 16, 2025
概括
热冲击反应 (HSR) 涉及蛋白质稳态和细胞存活. 在酵母菌中,核孔综合体和核篮蛋白质支架转录凝聚物,聚合HSF1点基因,并可能有助于疾病治疗.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 热冲击反应 (HSR) 是一种保存的细胞机制,用于维持蛋白质稳态和在压力下生存.
- 热冲击因子1 (HSF1) 是HSR的主调节者,诱导分子伴侣和恒温因子的基因.
- 高速转录过程 (HSR) 涉及可诱导的分相冷凝物形成,这些冷凝物集中转录机械.
研究的目的:
- 审查HSR中将基因组架构,转录凝聚物,核孔综合体 (NPC) 和核篮蛋白联系在一起的证据.
- 讨论这些发现对疾病治疗的影响.
主要方法:
- 关于热冲击反应,HSF1,转录凝聚物和核孔复合物的现有科学文献的审查.
- 对酵母模型研究的研究分析,研究在HSR期间的基因聚类和凝结物形成.
- 核篮蛋白和NPC支架蛋白在HSR诱导的基因组织中的作用的检查.
主要成果:
- 在酵母中,HSR涉及相分离的凝聚物,驱动HSF1基因之间的染色体内和染色体间相互作用,形成DNA循环.
- 核孔综合体 (NPC) 作为转录凝聚物形成和染色体组织的支架.
- 核篮蛋白,但不是必不可少的NPC支架蛋白,有助于热冲击诱导的HSF1位点的基因间聚类.
结论:
- 基因组架构,转录凝聚物和NPC在调节HSR方面相互连接.
- 核篮蛋白在压力期间在组织HSF1基因中发挥关键作用.
- 了解这些机制可能为疾病提供新的治疗策略.
相关概念视频
Chromatin Structure Regulates pre-mRNA Processing
8.1K
In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
The chromatin structure, especially...
8.1K
Chromatin Position Affects Gene Expression
24.6K
Chromatin is the massive complex of DNA and proteins packaged inside the nucleus. The complexity of chromatin folding and how it is packaged inside the nucleus greatly influences access to genetic information. Generally, the nucleus' periphery is considered transcriptionally repressive, while the cell's interior is considered a transcriptionally active area.
Topologically Associated Domains (TADs)
The 3-dimensional positioning of chromatin in the nucleus influences the...
Topologically Associated Domains (TADs)
The 3-dimensional positioning of chromatin in the nucleus influences the...
24.6K
Regulation of Nuclear Protein Sorting
3.2K
Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...
3.2K
Nucleosome Remodeling
10.8K
Nucleosomes are the basic units of chromatin compaction. Each nucleosome consists of the DNA bound tightly around a histone core, which makes the DNA inaccessible to DNA binding proteins such as DNA polymerase and RNA polymerase. Hence, the fundamental problem is to ensure access to DNA when appropriate, despite the compact and protective chromatin structure.
Nucleosome remodeling complex
Eukaryotic cells have specialized enzymes called ATP-dependent nucleosome remodeling enzymes. These enzymes...
Nucleosome remodeling complex
Eukaryotic cells have specialized enzymes called ATP-dependent nucleosome remodeling enzymes. These enzymes...
10.8K
Duplication of Chromatin Structure
7.2K
The process of chromosome duplication during cell division requires genome-wide disruption and re-assembly of chromatin. The chromatin structure must be accurately inherited, reassembled, and maintained in the daughter cells to ensure lineage propagation.
The basic unit of the chromatin is the nucleosome, consisting of DNA wrapped around octameric histone proteins and short stretches of linker DNA separating individual nucleosomes. The histone proteins within the nucleosome have their...
The basic unit of the chromatin is the nucleosome, consisting of DNA wrapped around octameric histone proteins and short stretches of linker DNA separating individual nucleosomes. The histone proteins within the nucleosome have their...
7.2K
Structure of a Gene
15.4K
A gene is the fundamental unit of heredity. Every individual has two copies of each gene, one inherited from each parent. Although most people contain the same genes, there is a small fraction that is slightly different amongst people. A gene with a small difference in its sequence of DNA bases forms different alleles, contributing to different phenotypes.
However, only 1% of the DNA is composed of genes that encode proteins; the rest, 99% is non-coding DNA. This non-coding DNA performs...
However, only 1% of the DNA is composed of genes that encode proteins; the rest, 99% is non-coding DNA. This non-coding DNA performs...
15.4K


