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Microbial Biosensors

Microbial Biosensors

Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...

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Updated: May 4, 2026

Quantification and Size-profiling of Extracellular Vesicles Using Tunable Resistive Pulse Sensing

Quantification and Size-profiling of Extracellular Vesicles Using Tunable Resistive Pulse Sensing

Published on: October 19, 2014

超材料传感器上的空间设计用于增强信号和检测细胞外囊泡.

Esma Derin^1,2, Eylul Gulsen Yilmaz^1,2, Özgecan Erdem¹

¹UNAM - National Nanotechnology Research Center, Bilkent University, Cankaya, Ankara 06800, Turkey.

ACS applied materials & interfaces

|October 16, 2025

概括

这项研究引入了新型的超材料等离子体生物传感器,使用重新设计的光盘进行经济高效的便携式细胞外囊泡检测. 创新的设计显著提高了对护理点诊断的敏感性.

关键词:

细胞外囊泡中的细胞外囊泡.超级材料传感器纳米岛的纳米岛是什么?光学磁盘是如何使用的信号增强信号增强信号增强

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Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets

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Published on: March 17, 2023

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Last Updated: May 4, 2026

Quantification and Size-profiling of Extracellular Vesicles Using Tunable Resistive Pulse Sensing

Quantification and Size-profiling of Extracellular Vesicles Using Tunable Resistive Pulse Sensing

Published on: October 19, 2014

Microfluidic Platform with Multiplexed Electronic Detection for Spatial Tracking of Particles

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Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets

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Published on: March 17, 2023

科学领域:

纳米技术和材料科学材料科学
生物医学工程生物医学工程
分析化学分析化学

背景情况:

生物传感器在分析性能,制造和成本方面面临挑战,这限制了它们在临床诊断 (POC) 中的使用.
基于元材料的等离子体生物传感器显示出希望,但受到灵敏度,复杂性和成本的阻碍.
现有的生物传感器平台往往缺乏便携性和实地应用的用户友好性.

研究的目的:

开发一种高度敏感,具有成本效益和便携性的超材料等离子体生物传感器,用于细胞外囊泡 (EV) 检测.
在超材料传感器上引入现场控制的空间设计,以提高分析性能.
将随时可用的光盘重新用作生物传感器制造的基板,降低成本和复杂性.

主要方法:

商业上可用的光盘被重新利用为纳米结构的基板,用于生物传感器制造.
在现场控制的空间设计和金纳米粒子 (AuNP) 或纳米岛 (NI) 工程被用来创建等离子热点.
用有限差异时间域 (FDTD) 模拟来分析近场效应并优化传感器设计.
通过纳米粒子跟踪分析 (NTA) 和光增强的NTA (fNTA) 检测出细胞外囊泡.

主要成果:

与e-beam光刻相比,重新使用的光盘基板将制造成本降低了高达260倍,时间降低了约960倍.
设计的等离子热点显著增强了局部电场,并将批量折射率灵敏度提高了多达5.5倍.
该平台的检测极限为10^4颗粒/μL (NTA),~330 fg/μL (EV质量) 和138 EVs/μL (fNTA).
开发了一个紧的,手掌大小的平台,提高了可用性和便携性.

结论:

重用光盘提供了一个可行的策略,以克服成本,复杂性和可用性障碍在超材料生物传感器开发.
开发的空间设计和等离子体热点工程为细胞外囊泡检测提供了一个高度敏感和易用的平台.
这种方法有可能推进医疗诊断和其他需要敏感生物标志物检测的各种应用.