相关实验视频

Updated: Jan 15, 2026

Microfluidic Tools for Probing Fungal-Microbial Interactions at the Cellular Level

Microfluidic Tools for Probing Fungal-Microbial Interactions at the Cellular Level

Published on: June 23, 2022

Fusarium solani 激活 PANoptosis 并调节真菌角膜炎中的免疫反应

Xiaoyuan Sha¹, Guocheng Yu², Changlin Wu²

¹Department of Ophthalmology, The Sixth Affiliated Hospital of Jinan University, Jinan University, Dongguan, China.

Investigative ophthalmology & visual science

|October 16, 2025

概括

在真菌角膜炎中,Fusarium solani感染和脱氧尼瓦醇 (DON) 触发了细胞死亡途径PANoptosis. 准这种途径为这种眼部感染提供了潜在的新疗法.

科学领域:

眼科医生眼科眼科
免疫学免疫学免疫学
菌类学菌类学是指菌类学.

背景情况:

性角膜炎是一种严重的眼部感染.
Fusarium solani是真菌角膜炎的常见原因之一.
细胞死亡途径 PANoptosis 在炎症性疾病中起作用.

研究的目的:

为了调查Fusarium solani如何在真菌角膜炎中触发PANoptosis.
为了检查脱氧尼瓦伦醇 (DON) 对人类角膜上皮细胞的毒性作用.
阐明DON诱导的细胞死亡和炎症的机制.

主要方法:

使用F. solani. 建立了一个真菌角膜炎小鼠模型.
在角膜组织中分析了PANoptosis标记物和炎症性细胞因子.
利用RNA测序来识别差异表达的基因和免疫细胞透.
暴露于人类角膜上皮细胞的DON,并评估了PANoptosis标记物和基因表达.

主要成果:

索拉尼菌感染激活了角膜中的PANoptosis,通过增加的切割形,GSDMD和p-MLKL表示.
观察到炎症性细胞因子 (IL-6,IL-1β,TNF-α) 的显著上调.
在角膜细胞中DON暴露也诱导了PANoptosis标记物和炎症性细胞因子.
RNA测序揭示了免疫反应和细胞外矩阵组织途径的改变.

结论:

在真菌角膜炎中,F. solani感染和DON诱导PANoptosis和炎症.
准PANoptosis信号通路为真菌角膜炎提供了一个新的治疗策略.

更多相关视频

Live Imaging of Antifungal Activity by Human Primary Neutrophils and Monocytes in Response to A. fumigatus

Live Imaging of Antifungal Activity by Human Primary Neutrophils and Monocytes in Response to A. fumigatus

Published on: April 19, 2017

Evaluation of Caspase Activation to Assess Innate Immune Cell Death

Evaluation of Caspase Activation to Assess Innate Immune Cell Death

Published on: January 20, 2023

相关概念视频

Fungal Phylum Microsporidia

Fungal Phylum Microsporidia

Microsporidia are a group of obligate intracellular fungi that were initially classified as protists but were later reclassified based on phylogenetic, molecular, and structural evidence linking them to the Chytridiomycota. These unicellular, non-motile organisms are highly specialized parasites that infect a wide range of animal hosts, including humans. They have evolved extensive genomic and metabolic reductions, making them highly dependent on their hosts for survival.Morphology and Genomic...

Fungal Group Zygomycota

Fungal Group Zygomycota

Zygomycota, previously classified as a distinct fungal group, are primarily terrestrial, saprophytic molds that play a crucial role as decomposers. Recent phylogenetic studies have revealed that these fungi are now divided into two major clades — Mucoromycota, which includes many symbiotic species, and Zoopagomycota, which primarily consists of parasitic and pathogenic fungi. These groups exhibit distinct ecological roles and reproductive strategies while sharing key structural and...