exoRBase 3.0:来自人类生物流体的细胞外RNA的更新资源
Wenqian Yu1,2, Jia Hu1,2, Peng Lin1,2
1Department of Integrative Oncology, Fudan University Shanghai Cancer Center, and Shanghai Key Laboratory of Medical Epigenetics, Institutes of Biomedical Sciences, Fudan University, Shanghai 200032, China.
Nucleic acids research
|October 16, 2025
概括
更新后的 exoRBase 3.0 数据库现在比较了来自细胞外囊泡和粒子 (EVPs) 和无细胞分数的细胞外长RNA (exLRs). 本资源有助于发现新的RNA生物标志物,以改善非侵入性疾病诊断和监测.
科学领域:
- 生物化学 生物化学
- 基因组学就是基因组学.
- 生物信息学是一种生物信息学.
背景情况:
- 生物流体中的细胞外RNA (exRNA) 是疾病检测的关键非侵入性生物标志物.
- 了解exRNAs的不同来源 (例如,囊泡结合与无细胞结合) 对于优化液体活检至关重要.
- 现有的数据库缺乏对不同细胞外RNA分量的全面比较分析.
研究的目的:
- 为了介绍exoRBase 3.0,一个扩展的数据库整合和比较细胞外长RNA (exLRs).
- 提供第一个大型平台,以区分与EVP相关的和无细胞的exLRs.
- 为了促进RNA表达,途径和起源的比较分析,以改进液体活检策略.
主要方法:
- 从细胞外囊泡和颗粒 (EVPs) 和无细胞分片的exLR数据的系统整合.
- 开发一个比较分析平台,区分EVP相关的和无细胞的exLRs.
- 包括 12,981 个基因组的交互可视化和扩展路径分析.
主要成果:
- exoRBase 3.0 整合了来自 2,913 个样本 (血液,尿液,脑脊液,胆汁) 的 19,927 个 mRNA, 15,961 个 lncRNA 和 126,816 个 circRNA.
- 该数据库允许从EVP和无细胞分数中大规模比较exLRs.
- 增强的途径分析有助于理解功能角色和组织起源.
结论:
- exoRBase 3.0 是一个有价值的资源,用于对不同生物流体分数的exLR进行比较分析.
- 本资源支持识别用于精密医学的特定环境RNA生物标志物.
- 这些发现促进了使用液体活检进行非侵入性疾病诊断,监测和治疗应用.
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