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通过MALDI空间映射神经异构体捕获的离子流动性MS成像MS成像

Samuel Okyem^1,2, Timothy J Trinklein^1,2, Stanislav S Rubakhin^1,2

¹Department of Chemistry, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, United States.

Analytical chemistry

|October 16, 2025

概括

研究人员开发了一种新的成像技术,用于在神经系统中绘制d-氨基酸含 (DAACPs) 的地图. 这种方法揭示了这些重要的神经及其神经元内的异构体的空间分布.

科学领域:

神经科学是一个神经科学.
分析化学分析化学
生物化学生物化学

背景情况:

含有D-氨基酸的 (DAACP) 是功能上重要的神经,但它们的空间分布尚不清楚.
现有的分析方法难以根据其氨基酸的性图像.

研究的目的:

开发和应用一种新的非向成像技术,用于神经类立体异构体.
绘制DAACP及其l-氨基酸对应物在中枢神经系统 (CNS) 的空间分布图.

主要方法:

使用矩阵辅助激光脱/电离捕获离子移动性质谱成像 (MALDI-TIMS-MSI) 进行非目标成像.
应用串联质谱法 (MS/MS) 用于异构体确认和消除异构体干扰.
在单细胞分辨率下分析了 *Aplysia californica* 的中枢神经系统.

主要成果:

成功地在*Aplysia californica*的中枢神经系统中绘制了神经及其DAACP形式,具有单细胞分辨率.
发现,虽然这两种立体异构体形式都在神经和神经中被局部化,但DAACPs在神经 soma中缺席.
从6个内源性神经中分辨出13个类立体同位素,包括小心脏活性B.

结论:

MALDI-TIMS-MSI是一个强大的工具,用于特征和映射*in situ*的类立体异构体.
提供了关于神经系统内神经异构的空间调节的关键见解.
促进了对神经元信号传递中的DAACP功能和分布的理解.

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