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模拟天然的四季发酵系统,用于制高盐稀释状态大豆:在调味促进法规中的应用
Changzheng Wu1,2,3,4, Hui Wu3, Xiya Yu3
1Key Laboratory of Advanced Technology Enterprise of Guangdong Seasoning Food Biofermentation, Foshan, China.
PloS one
|October 16, 2025
概括
豆风味的一致性受到季节性温度的挑战. 这项研究开发了一种模拟系统,以识别像Staphylococcus lloydii和Kodamaea ohmeri这样的关键微生物,这些微生物在低温发酵过程中增强了芳香化合物.
科学领域:
- 食品科学 食品科学 食品科学
- 微生物学 微生物学
- 发酵技术的发酵技术
背景情况:
- 季节性温度波动显著影响大豆的发酵,导致不一致的风味,特别是在较低的温度下.
- 微生物的代谢活动在低温 (例如15°C) 时受到限制,从而减少了芳香化合物.
- 传统的多年实地研究在优化工业大豆汁生产方面效率低下.
研究的目的:
- 开发和使用基于实验室的季节性温度模拟发酵系统来研究大豆生产中的微生物动态.
- 在不同温度条件下识别特定的微生物菌株及其在风味发展中的作用.
- 提供一种加速优化大豆发酵的方法,以全年保持一致的风味.
主要方法:
- 开发一个季节性温度模拟发酵系统,复制林南地区的气候条件 (15-37°C).
- 在不同温度下 (15°C与30°C) 进行大豆发酵的比较分析.
- 确定影响风味和气味的关键微生物菌株 (Staphylococcus lloydii,Leuconostoc lactis,Kodamaea ohmeri) 的鉴定.
- 在低温发酵中对特定菌株进行实验性注射,以评估它们对芳香化合物形成的影响.
主要成果:
- 在15°C的发酵 (冬季模拟) 与30°C (秋季模拟) 相比,导致大豆风味较弱.
- 在低温发酵大豆中观察到少量的Staphylococcus lloydii,Leuconostoc lactis和Kodamaea ohmeri. 在低温发酵大豆中观察到少量的Staphylococcus lloydii,Leuconostoc lactis和Kodamaea ohmeri.
- 在低温下对 Staphylococcus lloydii 和 Kodamaea ohmeri 的注射显著促进了关键芳香化合物的形成,如乙,1-octen-3-ol 和乙烯酸.
结论:
- 开发的模拟系统有效地复制季节性温度变化,以加速大豆发酵研究.
- 特定的微生物菌株,特别是 Staphylococcus lloydii 和 Kodamaea ohmeri,对于在低温下保持大豆的风味和香味至关重要.
- 这项研究为大豆行业提供了一种具有成本效益的工具,用于测试微生物菌株的重新排卵,确保全年保持一致的风味特征.
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