在Mycobacterium tuberculosis复合体内的病毒性等级
Sarah N Danchuk1,2,3, Shannon C Duffy4, Jaryd Sullivan5
1Department of Microbiology and Immunology, McGill University, Montreal, QC H3A 0G4, Canada.
概括
动物适应的Mycobacterium结核复合体 (MTBC) 菌株的毒力比M.结核更高. 病原性差异与特定的毒性因素和宿主免疫有关,影响了结核病研究.
科学领域:
- 微生物学 微生物学
- 免疫学 免疫学 免疫学
- 兽医医学 兽医医学 兽医医学
背景情况:
- 结核菌复合体 (MTBC) 包括结核菌 (M. tb),M. bovis和M. orygis,所有这些都对人类和动物健康构成重大威胁.
- 了解这些密切相关的MTBC成员之间的致病性差异对于疾病控制至关重要.
研究的目的:
- 为了比较评估M. tb,M. bovis和M. orygis的毒性.
- 确定有助于MTBC内差异性致病性的关键因素.
主要方法:
- 在牛 (荷尔斯坦小牛) 和小鼠 (C57BL / 6小鼠) 感染模型中通过气溶接种进行比较性毒性评估.
- 病理分析 (宏观,显微,细菌负担) 和生存研究.
- 基因破坏和蛋白质组分析以确定毒性因素.
主要成果:
- 适应动物的MTBCs (M. bovis,M. orygis) 在两种模型中都表现出与M. tb相比显著增强的毒性.
- 在被M. bovis或M. orygis感染的小鼠中观察到明显的免疫病理和快速死亡率 (24天内).
- 病毒性取决于共享 (ESAT-6) 和血统相关 (MPT70) 因素,感染途径和宿主免疫状态.
结论:
- 在MTBC中存在意想不到的毒性等级,动物适应的菌株更具毒性.
- 这些发现对结核病的研究和控制策略有着根本性的和翻译性的影响.
- 特定的毒性因素和宿主-病原体相互作用决定了MTBC的致病性.
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