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相关概念视频

Ribosome Profiling02:24

Ribosome Profiling

4.1K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
4.1K
Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

1.3K
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
1.3K
Transfer RNA Synthesis02:36

Transfer RNA Synthesis

13.2K
One of the unique features of tRNA is the presence of modified bases. In some tRNAs, modified bases account for nearly 20% of the total bases in the molecule. Altogether, these unusual bases protect the tRNA from enzymatic degradation by RNases.
Each of these chemical modifications is carried by a specific enzyme, post-transcription. All of these enzymes have unique base and site-specificity. Methylation, the most common chemical modification, is carried by at least nine different enzymes, with...
13.2K
RNA Editing02:23

RNA Editing

9.8K
RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
9.8K
Translational Regulation01:29

Translational Regulation

531
Translational regulation in prokaryotes ensures efficient protein synthesis by controlling ribosome access to mRNA. This regulation is mediated by secondary RNA structures, including translational riboswitches, RNA thermometers, and small RNAs (sRNAs), which respond to intracellular and environmental signals to modulate gene expression.Translational RiboswitchesRiboswitches in the leader region of mRNAs can regulate translation by altering the accessibility of the Shine-Dalgarno (SD) sequence,...
531
Riboswitches01:56

Riboswitches

9.5K
Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
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相关实验视频

Updated: Jan 15, 2026

Quantitative Immunofluorescence to Measure Global Localized Translation
09:13

Quantitative Immunofluorescence to Measure Global Localized Translation

Published on: August 22, 2017

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精确修改的核酸合成,以揭示修改对信息核酸翻译的特定效应

Yufan Pan1, Chenyou Zhu1, Yuan Zhuang1,2

  • 1Engineering Research Center of Advanced Rare Earth Materials (Ministry of Education), Department of Chemistry, Tsinghua University, Beijing, 100084, China.

Journal of the American Chemical Society
|October 16, 2025
PubMed
概括

这项研究引入了一种创建特定位点修改的长单链RNA (ssRNA) 的新方法. 这种进步允许精确控制RNA修饰,提高mRNA治疗的安全性和有效性.

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A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues
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A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues

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A Rapid High-throughput Method for Mapping Ribonucleoproteins RNPs on Human pre-mRNA
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A Rapid High-throughput Method for Mapping Ribonucleoproteins RNPs on Human pre-mRNA

Published on: December 2, 2009

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相关实验视频

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Quantitative Immunofluorescence to Measure Global Localized Translation
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Quantitative Immunofluorescence to Measure Global Localized Translation

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A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues
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A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues

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A Rapid High-throughput Method for Mapping Ribonucleoproteins RNPs on Human pre-mRNA
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科学领域:

  • 生物化学
  • 分子生物学
  • 药物开发

背景情况:

  • 对于mRNA疗法开发来说,RNA的修改至关重要.
  • 对于特定地点的修改准确性和生物安全性存在担忧.
  • 了解精确的修饰效应对于推进mRNA疗法至关重要.

研究的目的:

  • 开发一种以模板为导向的策略,用于合成特定位置的长ssRNA.
  • 研究精确修改的mRNA模式对翻译的影响.
  • 为下一代疗法和RNA表观遗传学提供mRNA修饰指南.

主要方法:

  • 开发了长ssRNA (超过300nT) 的模板导向合成策略.
  • 实现了单基分辨率修改,包括m6A,m5C,m1Ψ, Ψ,I,Br-dU,Cy3,Cy5,FAM,2'-F,2'-OMe,2'-MOE,2'-Propargyl,LNA,cET和PS.
  • 研究了特定修饰模式对mRNA翻译的功能影响.

主要成果:

  • 在单基分辨率下成功合成了具有特定位点修饰的长ssRNA.
  • 证明在特定位置的m1Ψ修改提高了翻译准确性.
  • 观察到m1Ψ修改保留了较低的免疫性,同时改善了翻译.

结论:

  • 开发的策略可以精确控制长ssRNA的RNA修饰.
  • 网站特定的m1Ψ修改提供了一种有前途的方法来提高mRNA翻译的真实性和安全性.
  • 这项研究为开发先进的mRNA药物和推进RNA表观遗传学提供了基础.