基于液体染色学-双重质谱的重新校准可以减少跨平台的变异性,在化学发光免疫测试平台上检测阿尔多
Jing Zhang1, Baobin Luo1, Zifu Fan1
1Department of Laboratory Medicine, Beijing Tongren Hospital, Capital Medical University, Beijing, China.
Clinica chimica acta; international journal of clinical chemistry
|October 16, 2025
概括
精确的阿尔多素 (ALD) 测量对于管理原发性阿尔多素 (PA) 症至关重要. 这项研究在CLIA平台和LC-MS/MS测定中发现了显著的不一致性,突出了改善临床ALD测量的标准化校准的必要性.
科学领域:
- 临床化学 临床化学
- 免疫测试技术 免疫测试技术
- 质谱测量质量谱测量
背景情况:
- 精确的阿尔多素 (ALD) 测量对于诊断和管理原发性阿尔多素 (PA) 症至关重要.
- 化学发光免疫测试 (CLIA) 平台之间的跨平台变异性阻碍了可靠的临床决策.
- 这项研究解决了改善ALD测试标准化的需要.
研究的目的:
- 为了比较六个CLIA平台和一个液体染色学-并联质谱法 (LC-MS/MS) 试验的性能,用于检测阿尔多素.
- 评估使用LC-MS/MS重新校准策略的有效性,以提高CLIA平台之间的一致性.
- 确定减少临床ALD测量的跨平台变异性的策略.
主要方法:
- 在50个临床血样本中测量了阿尔多的水平,这些样本在7个测试 (六个CLIA,一个LC-MS/MS) 中进行了聚合.
- 统计分析包括弗里德曼测试,斯皮尔曼相关性,传递-巴布洛克回归和布兰德-阿尔特曼分析.
- 根据LC-MS/MS重新校准了CLIA平台,并在重新校准前后评估一致性.
主要成果:
- 与CLIA平台相比,LC-MS/MS始终产生较低的ALD水平 (P <0.05).
- 虽然观察到强烈的相关性 (R ≥0.955),但回归斜率和截止的显著偏差表明偏差.
- 再校准减少了平均差异,但在纠正比例和系统偏差方面取得了有限的成功.
结论:
- 在CLIA平台和LC-MS/MS测定之间存在低于最佳的一致性.
- 标准化的校准策略对于最大限度地减少临床ALD测试中的平台间变化至关重要.
- 需要进一步优化校准和检测方法,以解决持久偏差.
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