在肝细胞癌中,RNF216P1通过调节miR-195-5p/ATG4B轴作为致癌基因起作用
Kai Li1, Yaping Bai1, Jingtong Wang1
1Anhui Province Key Laboratory of Basic Research and Transformation of Age-related Diseases, 22 Wenchang West Road, Wuhu City, Anhui Province, 241002, China.
长非编码RNARNF216P1在肝细胞癌 (HCC) 中被上调,促进瘤生长. 它充当miR-195-5p的竞争RNA (ceRNA),增加ATG4B水平并增强自,为HC.CC.提供潜在的治疗点.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 长非编码RNAs (lncRNAs) 越来越多地被认为是它们在癌症发展中的作用.
- 肝细胞癌 (HCC) 是一个主要的全球健康问题,具有复杂的分子基础.
- 了解参与HCC进展的特定lncRNA对于确定新的治疗策略至关重要.
研究的目的:
- 研究lncRNA RNF216P1在肝细胞癌 (HCC) 中的作用和机制.
- 阐明涉及RNF216P1,miR-195-5p和ATG4B在HCC进展中的分子轴.
- 确定RNF216P1是否可以作为HCC的潜在治疗点.
主要方法:
- 对癌症基因组图谱 (TCGA) 数据集对RNF216P1,miR-195-5p和HCC中的ATG4B表达的分析.
- 基因和蛋白质表达的验证使用RT-qPCR和西式涂抹.
- 在体外和体外功能测定包括细胞计数套件-8 (CCK-8),伤口愈合,Transwell迁移和异种移植模型.
- 使用光在位杂交 (FISH),RNA免疫沉降 (RIP) 和双露西法酶记者测定来确认RNA相互作用.
主要成果:
- 发现RNF216P1在HCC组织中显著上调,并促进瘤生长.
- RNF216P1充当了对 miR-195-5p 的竞争性内源RNA (ceRNA).
- 这种相互作用导致了与自相关的4B胺酶 (ATG4B) 的上调,并增强了自,促进了HCC恶性病变.
结论:
- 通过调节RNF216P1/miR-195-5p/ATG4B ceRNA轴,RNF216P1促进了HCC的进展.
- 已识别的ceRNA网络在HCC发育中起着至关重要的作用.
- RNF216P1代表了肝细胞癌的一个潜在的新型治疗点.
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