斑马鱼对甲酸盐暴露和恢复的反应:洞察基于转录基因组的分子机制
Jinbing Dai1, Haiyue Jing1, Shiguo Li2
1MOE Key Laboratory of Resources and Environmental Systems Optimization, College of Environmental Science and Engineering, North China Electric Power University, Beijing, 102206, China.
Environmental pollution (Barking, Essex : 1987)
|October 16, 2025
概括
酸 (PAE) 在斑马鱼中引起持续的毒性,即使在去除后. 分子损伤在恢复过程中会恶化,这凸显了在风险分析中进行暴露后评估的必要性.
科学领域:
- 环境毒理学环境毒理学
- 水生物质毒理学研究
- 分子毒理学分子毒理学
背景情况:
- 酸乙 (PAE) 是水生环境中常见的可塑剂.
- 已知PAE对水生生物有毒,但回收和机制尚不清楚.
- 对PAE的准确风险评估受到其毒性影响和恢复方面的知识差距的限制.
研究的目的:
- 研究斑马鱼中PAE毒性和恢复的分子机制.
- 评估PAE影响在暴露停止后的持续性.
- 为改善PAE环境风险评估提供见解.
主要方法:
- 斑马鱼被暴露在二甲基甲酸盐 (DMP),二甲基甲酸盐 (DBP) 和二甲基甲酸盐 (DNOP) 中4天.
- 暴露后,在没有PAE的水中进行了为期7天的恢复期.
- 使用了积累评估,生存分析和转录组测序.
主要成果:
- 在暴露和恢复期间,在斑马鱼中检测到PAE.
- 转录组分析显示了明显的分子变化:DMP影响了PPAR信号传递和三甲代谢;DBP影响了谷甲代谢;DNOP抑制了细胞周期和DNA复制基因.
- 在恢复期间,分子变化加剧,表明持续和化合物特异性毒性.
结论:
- 在斑马鱼中,PAE暴露会诱导持续的,化合物特定的转录组毒性.
- 在恢复阶段分子损伤恶化,即使在PAE移除.
- 暴露后评估对于准确的毒理学研究和PAE风险评估至关重要.
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