由RNase P生成的tRFSer-GCT通过Adrb2信号传递促进脂肪细胞中的脂肪储存
Linyuan Shen1, Yuhang Lei1, Xue Zhao2
1Farm Animal Germplasm Resources and Biotech Breeding Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, China; State Key Laboratory of Swine and Poultry Breeding Industry, Sichuan Agricultural University, Chengdu, China; Key Laboratory of Livestock and Poultry Multi-omics, Ministry of Agriculture and Rural Affairs, College of Animal and Technology, Sichuan Agricultural University, Chengdu, China.
The Journal of biological chemistry
|October 16, 2025
概括
线粒体tRNA片段 (tRFs) 调节脂肪细胞的生长. 一种特定的tRF,tRFSer-GCT,通过准腺体受体Beta2来促进脂肪生成和脂质积累,揭示了脂肪组织中的新型tsRNA作用.
科学领域:
- 分子生物学分子生物学
- 代谢调节 代谢调节 代谢调节
- 在RNA生物学,RNA生物学.
背景情况:
- 由tRNA衍生的小RNAs (tsRNAs) 越来越多地被认为是细胞代谢的关键调节者.
- 不同脂肪组织储存区内tsRNAs的特定功能在很大程度上仍未被定义.
- 脂肪组织生物学和代谢健康是显著研究兴趣的领域.
研究的目的:
- 研究 tsRNAs 在棕色和白色脂肪组织中的作用和机制.
- 为了确定参与脂肪生成和脂质代谢的特定tsRNAs.
- 阐明脂肪组织中 tsRNAs 影响的调节通路.
主要方法:
- 在不同小鼠脂肪组织库 (BAT,淋巴腺WAT,iWAT) 中对tsRNA表达的分析.
- 使用3T3-L1细胞进行体外研究,以评估tRFs对脂肪生成和脂质积累的功能影响.
- 机理学研究涉及基因向,RNA免疫沉降,以及在小鼠模型中的体内救援实验.
主要成果:
- 一种特定的线粒体tsRNA,tRFSer-GCT,在棕色脂肪组织 (BAT) 中得到丰富,与线粒体丰富度相关.
- tRFSer-GCT在体外促进了脂肪生成和脂质积累,并局部化到线粒体.
- tRFSer-GCT生物发生取决于Ribonuclease P复合体,特别是Hsd17b10,它准腺核受体β2,降低其表达.
结论:
- tRFSer-GCT是一种关键的线粒体tsRNA,促进脂肪生成和脂肪组织中的脂质代谢.
- 核糖酶P - tRFSer-GCT - 腺核受体β2轴代表了脂肪组织生物学中的新型调节途径.
- 这些发现突出了tsRNAs作为脂肪组织内代谢调节的重要参与者.
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