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UDPG/P2Y-STAT1轴介导LPS诱导的反应性星化

Meng-Yao Wang¹, Yuan-Hong Deng¹, Xiao-Feng Ran¹

¹International Joint Research Centre on Purinergic Signalling/School of Health and Rehabilitation, Chengdu University of Traditional Chinese Medicine, Chengdu, 611137, China.

Neuropharmacology

|October 16, 2025

概括

星球细胞表达功能性P2Y14受体,调节神经炎症. 准这种受体通路为中枢神经系统炎症状况提供了一种新的治疗策略.

科学领域:

神经科学是一个神经科学.
免疫学免疫学免疫学
细胞生物学细胞生物学

背景情况:

星球细胞在中枢神经系统 (CNS) 神经炎症中至关重要.
纯能G蛋白结合受体P2Y14与神经炎症有关,但其在星球细胞中的作用尚不清楚.
了解星体P2Y14的功能对于神经炎症研究至关重要.

研究的目的:

为了研究P2Y14受体表达和分布在星球细胞中.
阐明P2Y14在星细胞炎症反应中的调节作用.
探索涉及UDPG/P2Y14-STAT1信号轴的潜在分子机制.

主要方法:

使用初级小鼠星体细胞和C8-D1A细胞系.
免疫光,西部斑点和RT-qPCR证实了P2Y14的表达.
药理学剂 (PPTN,UDPG) 和lentiviral转导调节P2Y14的活性和表达.

主要成果:

P2Y14受体在星球细胞中表达,它们的表达在LPS刺激时会增加.
通过UDPG激活P2Y14,促进星球细胞激活 (GFAP表达) 和炎症媒介释放 (IL-6,IL-18,TNF-α).
抗 P2Y14 抗体 PPTN 抑制 GFAP 表达和炎性细胞因子释放,部分通过 STAT1/p-STAT1 途径.

结论:

星球细胞表达功能性P2Y14受体,在神经炎症中发挥关键作用.
UDPG/P2Y14-STAT1信号通路是天体细胞炎症反应的关键调节者.
P2Y14代表了中枢神经系统炎症性疾病的潜在治疗标.

关键词:

互乐金-6是其中的一种.脂聚糖类糖化物脂聚糖类糖化物 P2Y(14) 的受体受体.反应性星球化是反应性的.信号传感器和转录激活器 1 1 1

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