在单个和共同培养的细胞系中比较石榴叶提取物和衍生代谢物的抗氧化活性
Federica Foglietta1, Giulia Querio2, Cinzia Sanna3
1Department of Drug Science and Technology, University of Turin, Via Pietro Giuria 9, 10125, Turin, Italy.
Scientific reports
|October 16, 2025
概括
石榴叶提取物及其化合物 (酸,醇) 提供抗氧化保护. 提取物在共同培养的细胞中显示出显著的二次抗氧化活性,增强抗氧化基因表达.
科学领域:
- 细胞生物学 细胞生物学
- 生物化学 生化学
- 药理学 药理学是指药理学的学科.
背景情况:
- 细胞氧化还原稳定对于生理功能至关重要,由抗氧化剂维持.
- 植物提取物提供外源抗氧化剂,对于对抗氧化应激至关重要.
- 红枣 (Punica granatum L.) 的叶子含有具有潜在抗氧化特性的生物活性化合物.
研究的目的:
- 研究石榴叶中乙醇总提取物 (TE) 和特定化合物 (酸/EA,醇/LU) 的抗氧化潜力.
- 评估它们在氧化应激下对人类皮肤纤维细胞 (HDF) 和人类静脉内皮细胞 (HUVEC) 的影响.
- 评估细胞活力,反应性氧物种生成和抗氧化基因表达.
主要方法:
- 测试了TE,EA和LU作为主要或次要抗氧化剂,以抵抗过氧化引起的氧化应激.
- 使用单细胞系培养 (HDF,HUVEC) 和共同培养的HDF-HUVEC模型.
- 评估了细胞活力,活性氧物种 (ROS) 水平和抗氧化基因的mRNA表达 (CAT,GPX1,NFE2L2,NOQ1,SOD1).
主要成果:
- 素 (LU) 和基酸 (EA) 分别在HDF和HUVEC细胞中表现出显著的初级抗氧化活性.
- 乙醇总提取物 (TE) 在HDF-HUVEC共同培养模型中表现出显著的二次抗氧化活性.
- 在共同培养的细胞中,TE治疗强烈上调了GPX1和NOQ1基因表达,与单细胞系中SOD1中度增加不同.
结论:
- 石榴叶提取物及其成分具有不同的抗氧化活性,取决于细胞类型和实验条件.
- 共同培养细胞试验突显了石榴叶提取物在减轻氧化应激诱导损伤方面的有效性.
- 石榴叶提取物在共同培养系统中有效调节抗氧化基因表达,特别是GPX1和NOQ1.
关键词:
普尼卡·格拉纳图姆 (Punica granatum L.) 的叶子提取物抗氧化剂是一种抗氧化剂.埃拉基酸是什么意思 埃拉基酸是什么意思卢特奥林是什么? 卢特奥林是什么?氧化应激是一种氧化应激.更多相关视频
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