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植物根和微生物的无标签结构成像使用第三和代显微镜

Daisong Pan¹, Jose A Rivera¹, Max Miao²

¹Department of Physics, University of California, Berkeley, CA, 94720, USA.

Scientific reports

|October 16, 2025

概括

研究人员开发了一种新的成像技术,使用微型制造生态系统 (EcoFAB) 和非线性多模式显微镜在现场可视化活植物根和微生物. 这种方法允许高分辨率,无标签观察根结构及其与树根球微生物的相互作用.

科学领域:

植物生物学植物生物学
微生物学微生物学
显微镜的使用方法

背景情况:

根系生物学对农业和气候变化减缓至关重要,但由于成像挑战,其研究仍然不足.
现有的方法难以在自然环境中可视化根结构和微生物相互作用.

研究的目的:

开发和应用一种新的,无标签的成像技术,用于植物根和根球微生物的现场可视化.
为了实现根结构和根微生物相互作用的高时空分辨率成像.

主要方法:

利用微型制造生态系统 (EcoFAB) 来创建具有光学接入的受控生长环境.
采用非线性多模式显微镜,特别是第三子生成 (THG) 和三光子光 (3PF).
实现了高分辨率的活根和微生物的无标签,现场成像.

主要成果:

通过THG显微镜可视化了关键的根结构 (血管,卡斯巴条,干干,根盖) 和亚细胞特征 (核包裹,核细胞,粉颗粒).
无标签的THG对比性在树叶球中的细菌和真菌细胞壁上得到了实现.
同时的3PF成像使单个细菌的追踪和真菌子和的亚细胞可视化成为可能.

结论:

开发的THG和3PF显微镜技术为根生物学提供了前所未有的无标签,现场成像能力.
这种方法促进了根系结构和根系中复杂的根系微生物相互作用的详细调查.
生态FAB与先进显微镜相结合,为推进植物和微生物研究提供了一个强大的平台.

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