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相关概念视频

Flow Cytometry01:23

Flow Cytometry

15.7K
The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...
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Proteomics01:33

Proteomics

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A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
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相关实验视频

Updated: Jan 14, 2026

Sample Preparation for Mass Cytometry Analysis
06:28

Sample Preparation for Mass Cytometry Analysis

Published on: April 29, 2017

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导航基于细胞计测的单细胞蛋白质组学的数据处理.

Huaicheng Sun1,2, Yuan Zhou1, Ruoyu Jiang1,3

  • 1College of Pharmaceutical Sciences, National Key Laboratory of Advanced Drug Delivery and Release Systems, Zhejiang University, Hangzhou, China.

Nature protocols
|October 16, 2025
PubMed
概括
此摘要是机器生成的。

安佩拉是一个新的工具,可以选数千个蛋白质组数据处理工作流程,以找到单细胞蛋白质组学 (SCP) 研究的最佳工作流程. 它有助于研究人员识别细胞亚群和轨迹,使复杂的生物数据分析更容易.

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Single-Cell Proteomics Preparation for Mass Spectrometry Analysis Using Freeze-Heat Lysis and an Isobaric Carrier
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Single-Cell Proteomics Preparation for Mass Spectrometry Analysis Using Freeze-Heat Lysis and an Isobaric Carrier

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Isolation and Staining of Mouse Skin Keratinocytes for Cell Cycle Specific Analysis of Cellular Protein Expression by Mass Cytometry
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Isolation and Staining of Mouse Skin Keratinocytes for Cell Cycle Specific Analysis of Cellular Protein Expression by Mass Cytometry

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相关实验视频

Last Updated: Jan 14, 2026

Sample Preparation for Mass Cytometry Analysis
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Sample Preparation for Mass Cytometry Analysis

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Single-Cell Proteomics Preparation for Mass Spectrometry Analysis Using Freeze-Heat Lysis and an Isobaric Carrier
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Single-Cell Proteomics Preparation for Mass Spectrometry Analysis Using Freeze-Heat Lysis and an Isobaric Carrier

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Isolation and Staining of Mouse Skin Keratinocytes for Cell Cycle Specific Analysis of Cellular Protein Expression by Mass Cytometry
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Isolation and Staining of Mouse Skin Keratinocytes for Cell Cycle Specific Analysis of Cellular Protein Expression by Mass Cytometry

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科学领域:

  • 蛋白质组学是指蛋白质组学.
  • 计算生物学 计算生物学
  • 数据科学数据科学数据科学

背景情况:

  • 单细胞蛋白质组学 (SCP) 提供了高分辨率的生物学见解.
  • 处理SCP数据涉及许多工作流程,使最佳选择具有挑战性.
  • 确定有效的数据处理策略对于强大的SCP分析至关重要.

研究的目的:

  • 开发ANPELA,一种用于大规模选SCP数据处理工作流程的新方法.
  • 为了能够比较数千个工作流程来识别细胞子群和伪时间轨迹.
  • 为优化SCP数据分析提供一个用户友好的,可访问的工具.

主要方法:

  • 安佩拉采用机器学习来大规模选蛋白质组数据处理工作流程.
  • 它比较了细胞亚群识别和伪时间轨迹推断中的工作流表现.
  • 部署了一个包,用于多场景可用性 (桌面,R,在线) 和数据安全.

主要成果:

  • ANPELA成功地确定了基于细胞计学的SCP研究的最佳数据处理工作流.
  • 该工具有助于比较成千上万的加工管道.
  • 案例研究表明ANPELA在导航复杂的蛋白质组数据方面的有效性.

结论:

  • 安佩拉为导航蛋白质组数据处理提供了一种现成的解决方案.
  • 它可供广泛的受众访问,包括非编码人员,增强SCP研究.
  • 该工具免费提供,促进更广泛的采用和数据驱动的生物发现.