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Updated: Jun 19, 2026

Visualization of Protein-protein Interaction in Nuclear and Cytoplasmic Fractions by Co-immunoprecipitation and In Situ Proximity Ligation Assay
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在现场近距离结合试验用于分析状蛋白之间的空间相互作用

Thorsten Pfirrmann1, Ulrich Rüther2, Christoph Gerhardt3,4

  • 1Institute of Molecular Medicine, Department of Medicine, Health and Medical University Potsdam, 14471, Potsdam, Germany.

BMC molecular and cell biology
|October 16, 2025
PubMed
概括

在位近距离结合试验 (in situ PLA) 检测到毛中的蛋白质近距离,有助于毛病研究. 这种方法绘制了子腔区内的蛋白质相互作用图,这对于理解腔功能和疾病至关重要.

关键词:
西里亚 (Cilia) 是一个小体.纤维病症是什么? 纤维病症是什么?光显微镜的光学显微镜.在现场近距离结合试验试验.蛋白质相互作用的相互作用滚动圈放大器 滚动圈放大器在Rpgrip1l.亚细胞局部化的局部化.过渡区的过渡区是一个过渡区.

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Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...