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开发和验证大西洋鱼 (Hippoglossus hippoglossus) 70K SNP 基因型阵列

Valentina Krivenjeva Sinani^1,2, Robert C Wilson³, Torfinn Nome⁴

¹Department of Biotechnology, CRESCO, Centre for Embryology and Healthy Development, University of Inland Norway, Hamar, Norway.

|October 16, 2025

概括

研究人员开发了大西洋鱼的第一个中密度单核酸多态 (SNP) 阵列. 这种新工具支持对这一重要的水产养殖物种进行基因组选择和遗传研究.

科学领域:

水产养殖的基因组学
鱼类育种技术鱼类育种技术
海洋生物技术海洋生物技术

背景情况:

大西洋鱼 (Hippoglossus hippoglossus) 缺乏必要的中密度基因型化工具,阻碍了基因组选择和遗传特征分析.
不断增长的水产养殖行业需要基因洞察力,以便明智地选择育种候选人和基因改进.
现有的基因组资源不足以进行大西洋鱼的高分辨率遗传研究.

研究的目的:

开发和描述了大西洋鱼的第一个中密度单核酸多态化 (SNP) 基因型阵列.
为基因组选择和这种物种的高分辨率遗传研究提供一个强大的工具.
促进基因改进和了解大西洋鱼在经济上重要的特征.

主要方法:

来自40个个人的全基因组测序 (WGS) 数据被用于变体发现.
一个定制的Affymetrix Axiom阵列设计了70,824个SNP测试,其中697个用于遗传性别确定.
该阵列通过对1152个大西洋鱼样本进行基因定型验证,评估呼叫率和基因型一致性.

主要成果:

超过800万个遗传变异被确定,包括6,224,375个SNP和2,131,436个插入删除变异 (INDELS).
开发的SNP阵列具有59,491个强大的SNP位点 (84%的总试验) 具有高性能 (99.64%的调用率,99.0%的一致性).
133个SNP测定被确定为在大西洋鱼中分配遗传性别的有效方法.

结论:

首个中密度SNP亚特兰大鱼基因型排序阵列已成功开发.
该阵列包含近6万个可靠的SNP测定,适用于全基因组研究和遗传性别确定.
这种阵列是高分辨率遗传研究和大西洋鱼实践基因组选择的宝贵平台.

关键词:

诊断性性别确定诊断性性别确定希波格洛索斯 (Hippoglossus) 是一个海马类动物.这是一个SNP-array.

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