Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

RNA-seq03:21

RNA-seq

11.7K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
11.7K
Ribosome Profiling02:24

Ribosome Profiling

4.1K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
4.1K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Engineered EV-mediated delivery of an anti-amyloid peptide provides neuroprotection in an in vitro Alzheimer's disease model.

International journal of pharmaceutics·2026
Same author

Human CLIC5 as a Recurrent Hotspot of HPV 16 Integration in Cervical Cancer.

Journal of medical virology·2026
Same author

Computational Discovery of NPA001937: A Novel Carotenoid Targeting Conserved Insect Proteins for Sustainable Pest Management.

Applied biochemistry and biotechnology·2026
Same author

An Overview of the Mechanisms of HPV-Induced Cervical Cancer: The Role of Kinase Targets in Pathogenesis and Drug Resistance.

Cancers·2026
Same author

Two-way transcriptome approach for the identification of common gene targets across four insect orders and its validation in Oxycarenus laetus.

Scientific reports·2025
Same author

miRNA dysregulation in Duchenne muscular dystrophy comorbidities.

World journal of experimental medicine·2025

相关实验视频

Updated: Jan 14, 2026

Rup (RNA-seq Usability Assessment Pipeline) - Quality Control for Bulk RNA-seq Experiments in Eukaryotes
05:07

Rup (RNA-seq Usability Assessment Pipeline) - Quality Control for Bulk RNA-seq Experiments in Eukaryotes

Published on: November 7, 2025

340

LncRAnalyzer:使用RNA-Seq进行长时间非编码RNA发现的强大工作流程.

Shinde Nikhil1, Habeeb Shaik Mohideen2, Raja Natesan Sella1

  • 1Membrane Protein Interaction Lab, Department of Genetic Engineering, SRM Institute of Science and Technology, Chengalpattu District, Tamil Nadu, 603203, India.

The Plant journal : for cell and molecular biology
|October 17, 2025
PubMed
概括
此摘要是机器生成的。

LncRAnalyzer是一种新的自动化管道,用于识别长非编码RNA (lncRNAs). 与现有方法相比,它提高了准确性,减少了错误,有助于植物研究.

关键词:
一个LncR分析仪在RNA-Seq.Sorghum 双色球是一种多彩的植物.基因组学就是基因组学.长非编码RNA是什么意思

更多相关视频

Author Spotlight: AQRNA-seq Role in Mapping Small RNAs and Unraveling Protein Translation Mechanisms
05:12

Author Spotlight: AQRNA-seq Role in Mapping Small RNAs and Unraveling Protein Translation Mechanisms

Published on: February 2, 2024

1.3K
A Bioinformatics Pipeline for Investigating Molecular Evolution and Gene Expression using RNA-seq
07:09

A Bioinformatics Pipeline for Investigating Molecular Evolution and Gene Expression using RNA-seq

Published on: May 28, 2021

10.4K

相关实验视频

Last Updated: Jan 14, 2026

Rup (RNA-seq Usability Assessment Pipeline) - Quality Control for Bulk RNA-seq Experiments in Eukaryotes
05:07

Rup (RNA-seq Usability Assessment Pipeline) - Quality Control for Bulk RNA-seq Experiments in Eukaryotes

Published on: November 7, 2025

340
Author Spotlight: AQRNA-seq Role in Mapping Small RNAs and Unraveling Protein Translation Mechanisms
05:12

Author Spotlight: AQRNA-seq Role in Mapping Small RNAs and Unraveling Protein Translation Mechanisms

Published on: February 2, 2024

1.3K
A Bioinformatics Pipeline for Investigating Molecular Evolution and Gene Expression using RNA-seq
07:09

A Bioinformatics Pipeline for Investigating Molecular Evolution and Gene Expression using RNA-seq

Published on: May 28, 2021

10.4K

科学领域:

  • 基因组学就是基因组学.
  • 生物信息学是一种生物信息学.
  • 分子生物学分子生物学

背景情况:

  • 长非编码RNAs (lncRNAs) 是关键的RNA分子,缺乏蛋白质编码潜力.
  • 鉴定lncRNAs是具有挑战性的,因为它们的丰度较低和复杂的表达模式.
  • 从蛋白质编码转录区分lncRNAs需要复杂的过方法.

研究的目的:

  • 开发一个自动化生物信息学管道,LncRAnalyzer,用于60种物种的准确 lncRNA识别.
  • 通过将蛋白质编码或部分蛋白质编码转录的包含降至最低,提高lncRNA预测.
  • 为分析 lncRNA 起源提供一个全面的工具,包括来自植物中可转移元素 (TE) 的来源.

主要方法:

  • 开发了LncRAnalyzer,这是一个自动化管道,用于对60种物种进行重新训练的模型.
  • 在工作流程中采用了八种不同的方法来完善 lncRNA 识别.
  • 使用的RNA-Seq数据和已知的lncRNA/CDS序列进行了10倍的交叉验证.
  • 与lncPipe,lncEvo,lncRNA-注释和植物lncPipe对比的LncR分析仪.

主要成果:

  • 与标准模型和其他谷验证方法相比,LncRAnalyzer表现出卓越的性能.
  • 该管道有效地减少了非蛋白质编码转录 (NPCTs) 的识别.
  • 基准测试证实LncRAnalyzer比现有的管道更全面,更用户友好,更准确.
  • 工作流程成功地确定了来自各种植物可移植元素 (TE) 的 lncRNA起源.

结论:

  • LncRAnalyzer提供了一个强大而准确的解决方案,用于在各种物种中识别lncRNA.
  • 该管道简化了lncRNA注释和分析的复杂过程.
  • LncRAnalyzer是植物基因组学研究的一个有价值的工具,特别是用于研究TE衍生的lncRNAs.