Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

907
Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
907

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Integrated two-photon and photoacoustic microscopy for single-cell neurometabolic imaging.

Nature communications·2026
Same author

Toward autonomous robotic-assisted and microrobotic surgery.

Science advances·2026
Same author

Simultaneous regeneration of skin and bone in full-thickness cranial composite defects.

bioRxiv : the preprint server for biology·2026
Same author

A Multilabel Single Molecule Localization Microscopy Protocol for Investigation of Chromatin in the Dense Nuclear Environment.

Journal of visualized experiments : JoVE·2026
Same author

Machine-Learning-Based Automated Schlemm's Canal Volumetric Segmentation for Optical Coherence Tomography.

Chemical & biomedical imaging·2026
Same author

One chromatin, many structures: From ensemble contact maps to single-cell 3D organization.

Biophysical journal·2026

相关实验视频

Updated: May 2, 2026

3D Orbital Tracking in a Modified Two-photon Microscope: An Application to the Tracking of Intracellular Vesicles
11:28

3D Orbital Tracking in a Modified Two-photon Microscope: An Application to the Tracking of Intracellular Vesicles

Published on: October 1, 2014

10.6K

量子棒的并行三维跟踪使用偏振敏感光谱光子定位显微镜.

Biqin Dong1,2, Brian T Soetikno1, Xiangfan Chen2

  • 1Biomedical Engineering Department, Northwestern University, Evanston, Illinois 60208, United States.

ACS photonics
|October 17, 2025
PubMed
概括

我们开发了一种新的显微镜技术,以3D追踪微小的半导体纳米粒子. 这种先进的成像方法精确地跟踪量子棒 (QR) 和它们的特性,从而实现更好的生物研究.

关键词:
极对称成像技术的极对称成像技术量子棒是一种量子棒.单颗粒追踪器是一个单颗粒追踪器.频谱学是一种光谱学.超分辨率显微镜的显微镜.

更多相关视频

A Protocol for Real-time 3D Single Particle Tracking
10:16

A Protocol for Real-time 3D Single Particle Tracking

Published on: January 3, 2018

15.3K
Author Spotlight: Non-Invasive Imaging of Complex Bio-Structures Using Polarization-Sensitive Two-Photon Microscopy
05:54

Author Spotlight: Non-Invasive Imaging of Complex Bio-Structures Using Polarization-Sensitive Two-Photon Microscopy

Published on: September 8, 2023

1.7K

相关实验视频

Last Updated: May 2, 2026

3D Orbital Tracking in a Modified Two-photon Microscope: An Application to the Tracking of Intracellular Vesicles
11:28

3D Orbital Tracking in a Modified Two-photon Microscope: An Application to the Tracking of Intracellular Vesicles

Published on: October 1, 2014

10.6K
A Protocol for Real-time 3D Single Particle Tracking
10:16

A Protocol for Real-time 3D Single Particle Tracking

Published on: January 3, 2018

15.3K
Author Spotlight: Non-Invasive Imaging of Complex Bio-Structures Using Polarization-Sensitive Two-Photon Microscopy
05:54

Author Spotlight: Non-Invasive Imaging of Complex Bio-Structures Using Polarization-Sensitive Two-Photon Microscopy

Published on: September 8, 2023

1.7K

科学领域:

  • 生物物理学的生物物理.
  • 纳米技术 纳米技术
  • 光学显微镜的使用方法

背景情况:

  • 半导体纳米晶体,像量子棒 (QRs),由于其亮度和光稳定性,在生物研究中是有价值的光探头.
  • 它们的光学特性,包括发射光谱和极化,为机械特性和纳米环境提供了洞察力.
  • 现有的方法在3D中同时跟踪多个QR,并提供详细的光谱和偏振信息时面临局限性.

研究的目的:

  • 开发和验证一种新的显微镜技术,用于高精度,多参数跟踪单个半导体纳米晶体.
  • 为了同时获取量子棒的3D位置,光谱和极化状态.
  • 为了提高多个量子棒的并行跟踪的可靠性,用于研究复杂的生物动力学.

主要方法:

  • 开发了一种三维 (3D),极化敏感的光谱光子定位显微镜 (3D-Polar-SPLM).
  • 同时对单个量子棒 (QR) 进行3D跟踪,具有高空间,光谱和极化分辨率.
  • 利用特定粒子的光谱特征来改善异质群体的识别和跟踪.

主要成果:

  • 实现了8nm的横向定位精度和35nm的轴精度的个别QRs.
  • 获得的光谱分辨率为2nm,极化角度测量精度为8度.
  • 通过使用光谱配置文件来区分单个QRs,证明了改进的跟踪保真性.

结论:

  • 3D-Polar-SPLM技术为量子棒的实时,多参数分析提供了前所未有的能力.
  • 这种技术显著提高了同时跟踪多个QR的能力,克服了以前方法的局限性.
  • 开发的显微镜为研究生物系统中的实时分子动力学提供了新的途径.