准备和体外免疫活性红多糖脂质体的多糖脂质体
Qinfang He1, Riqing Cheng1, Jiahao Shi1
1Inner Mongolia Medical University, Hohhot, China.
Iranian journal of pharmaceutical research : IJPR
|October 17, 2025
概括
红多糖脂质体 (RRPL) 通过提高生物可用性来增强免疫细胞的激活. 这项研究优化了RRPL制备,并证明了它们在激活淋巴细胞,巨细胞和树突细胞方面的有效性.
科学领域:
- 药理学和免疫学 药理学和免疫学
- 生物技术和药物输送 生物技术和药物输送
背景情况:
- 红多糖 (RRP) 具有免疫调节特性,但由于生物可用性差,治疗用途受到限制.
- 优化RRP交付对于提高其治疗疗效至关重要.
研究的目的:
- 优化使用直角实验设计制备Rosa rugosa多糖脂质体 (RRPL) 的过程.
- 评估开发的RRPL的体外免疫细胞激活能力.
主要方法:
- 使用逆相蒸发方法进行了RRP的脂质体封装.
- 使用正交测试来优化制备参数,重点是封装效率 (EE).
- 鉴定包括形态,颗粒大小,PDI,泽塔潜力,释放率和稳定性,随后对各种免疫细胞进行体外免疫学测试.
主要成果:
- 优化的RRPL实现了高EE (~81.96%) 和药物负载 (DL) (~13.86%) 与有利的粒子大小 (124.00 nm) 和稳定性.
- 与RRP相比,RRPL显示持续释放,改善生物可用性和延长作用.
- 观察到淋巴细胞增殖,巨细胞化,细胞因子分泌 (IL-6,IL-1β,IFN-γ) 和树突细胞激活 (IL-12p70,TNF-α) 的显著改善.
结论:
- 成功开发了优化的RRPL配方,具有增强的免疫细胞激活特性.
- RRPL代表了RRP的一个有前途的脂质体输送系统,提供了更好的治疗潜力.
- 这些发现为进一步研究和开发脂质体RRP用于免疫调节提供了基础.
相关概念视频
Cell-mediated Immune Responses
Overview
Insulin Secretory Vesicles
Insulin secretory vesicles release insulin to stimulate blood glucose uptake and regulate carbohydrate metabolism. When the blood glucose levels increase, glucose enters the pancreatic β-islet cells through glucose transporters. Once inside, glucose is metabolized through glycolysis, the citric acid cycle, and the electron transport chain, producing ATP. This increase in ATP concentration closes ATP-sensitive potassium channels, leading to depolarization of the membrane and the opening of...
Antigens Involved in Adaptive Immunity
An antigen is any substance the immune system identifies as foreign and potentially harmful to the body, prompting an immune response. Antigens have two functional properties: immunogenicity and reactivity. Immunogenicity is the ability of an antigen to stimulate a specific immune response. At the same time, reactivity describes the antigen's ability to react with the cells and antibodies produced in response to it.
Complete Antigens
Complete antigens possess both immunogenicity and reactivity.
Complete Antigens
Complete antigens possess both immunogenicity and reactivity.
Glucose Homeostasis: Pancreatic Islets and Insulin Secretion
The pancreatic islets comprising only 1%-2% of the volume are highly vascularized and innervated mini-organs. They contain five endocrine cell types, including β cells that secrete insulin, which is synthesized as a single polypeptide chain, preproinsulin, processed to proinsulin, and finally to insulin and C-peptide. This process is complex and regulated, involving the Golgi complex, the endoplasmic reticulum, and the secretory granules of the β cell.
Insulin and C-peptide are co-secreted in...
Insulin and C-peptide are co-secreted in...
Diabetes Mellitus: Introduction
Diabetes mellitus consists of chronic metabolic disorders characterized by persistent hyperglycemia. This elevated blood glucose results from defects in insulin secretion, impaired insulin action, or both. Insulin, produced by pancreatic β-cells, is essential for maintaining glucose homeostasis by facilitating cellular glucose uptake for energy or storage. Disruptions in insulin production or function lead to glucose accumulation in the bloodstream, causing the clinical features and long-term...
Hyperglycemia
Hyperglycemia is an abnormally high blood glucose level. It is diagnosed by fasting glucose ≥126 mg/dL, 2-hour oral glucose tolerance test (or OGTT) ≥200 mg/dL, random glucose ≥200 mg/dL with symptoms, or HbA1c ≥6.5%. However, HbA1c results may be unreliable in certain conditions, such as anemia or hemoglobinopathies, and the diagnosis should be confirmed unless classic symptoms are present. Postprandial hyperglycemia is typically considered significant when glucose levels exceed 180 mg/dL two...


