相关实验视频

Updated: Jan 14, 2026

Network Pharmacology Prediction and Experimental Validation of Trichosanthes-Fritillaria thunbergii Action Mechanism Against Lung Adenocarcinoma

Network Pharmacology Prediction and Experimental Validation of Trichosanthes-Fritillaria thunbergii Action Mechanism Against Lung Adenocarcinoma

Published on: March 3, 2023

整合网络药理和实验验证,以确定肝癌的关键草药成分和标

Fang Wang^1,2,3, Shenghao Li³, Xiling Liu¹

¹Hebei Medical University, Shijiazhuang, China.

Iranian journal of pharmaceutical research : IJPR

|October 17, 2025

概括

传统中医药 (TCM) 在肝癌治疗方面表现有前途. 这项研究确定了关键的草药,比如β-醇等化合物,以及JUN等标,通过网络药理学和体外实验验验证了它们的治疗潜力.

科学领域:

综合性瘤学是一种综合性瘤学.
网络药理学网络药理学
计算生物学是一种计算生物学.

背景情况:

传统中医 (TCM) 提供了一种整体的策略来管理恶性瘤.
肝癌仍然是一个重大的全球健康挑战,需要新的治疗方法.

研究的目的:

用数据挖掘和网络药理学来预测高频草药,活性化合物和肝癌治疗的核心分子标.
建立一个全面的网络,将TCM,活性成分,标和肝癌联系起来.
通过分子对接和体外测试来验证已识别的组件和点的治疗潜力.

主要方法:

使用Cytoscape构建一个"TCM - 活性成分 - 目标 - 疾病"网络.
通过STRING数据库开发蛋白质与蛋白质相互作用 (PPI) 网络,用于核心目标识别.
使用AutoDock Vina和PyMOL进行分子对接模拟,以评估结合亲缘关系.
在体外实验中评估奎尔素对肝癌细胞迁移,细胞亡和蛋白质表达的影响.

主要成果:

识别了50种高频TCM,包括*Atractylodes macrocephala*,*Astragalus membranaceus*,*Scutellaria barbata*和*Cremastra appendiculata*.这些高频TCM的确诊情况包括在内.
发现了226个常见的标,其中β-固醇,kaempferol,stigmasterol和luteolin被确定为潜在的核心成分.
对8个关键目标 (JUN,MAPK1,RELA,TNF,ESR1,IL-6,TP53,FOS) 的选,涉及417个入口和159个通道.
分子对接证实了强烈的结合亲缘关系;体外研究表明,奎尔素通过调节p-c-Jun/c-Jun和c-Fos表达来抑制HepG2细胞迁移和诱导细胞亡.

结论:

这项研究为TCM在肝癌治疗中的临床应用提供了数据驱动的基础.
确定了核心组件和目标,为肝癌管理提供了潜在的治疗策略.
网络药理学和实验验证强调了TCM在癌症治疗中的复杂机制.

关键词:

AP-1 AP-1 AP-1 AP-1 AP-1 AP-1 AP-1 AP-1 AP-1 AP-1 AP-1 AP-1 AP-1 AP-1 AP-1 AP-1 AP-1 AP-1 AP-1 AP-1 AP-1 AP-1 AP-1 AP-1 AP-1 AP-1 AP-1 AP-1 AP-1 AP-1 AP-1 AP-1 AP-1 AP-1 AP-1 AP-1 热清除 - 清除热量激发活力的人气.肝癌肝癌是一种癌症.传统中国医药传统中国医药

更多相关视频

Network Pharmacology Prediction and Metabolomics Validation of the Mechanism of Fructus Phyllanthi against Hyperlipidemia

Network Pharmacology Prediction and Metabolomics Validation of the Mechanism of Fructus Phyllanthi against Hyperlipidemia

Published on: April 7, 2023

Establishment of Hepatocarcinoma in BALB/c-nu Mice and Investigation of the Therapeutic Effect of the Sanleng Jiashen Formula

Establishment of Hepatocarcinoma in BALB/c-nu Mice and Investigation of the Therapeutic Effect of the Sanleng Jiashen Formula

Published on: January 26, 2024

相关概念视频

Targeted Cancer Therapies

Targeted Cancer Therapies

The targeted cancer therapies, also known as “molecular targeted therapies,” take advantage of the molecular and genetic differences between the cancer cells and the normal cells. It needs a thorough understanding of the cancer cells to develop drugs that can target specific molecular aspects that drive the growth, progression, and spread of cancer cells without affecting the growth and survival of other normal cells in the body.
There are several types of targeted therapies against...

Effect of Hepatic Disease on Pharmacokinetics: Pathophysiologic Assessment and Liver Function Test

Effect of Hepatic Disease on Pharmacokinetics: Pathophysiologic Assessment and Liver Function Test

In clinical practice, the direct measurement of hepatic blood flow to evaluate liver function presents significant challenges due to the intricate and specialized nature of the necessary techniques. Consequently, healthcare professionals often rely on empirical estimates derived from thorough patient examinations and liver function tests to gauge liver health. Among the tools at their disposal, the Child–Pugh and MELD scoring systems stand out for their ability to categorize and assess...