隔离来自胃癌腹膜转移的患者衍生的细胞和细胞外囊泡的协议
Yibo Fan1, Jody V Vykoukal2, Shumei Song3
1Department of GI Medical Oncology, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA.
STAR protocols
|October 17, 2025
概括
这项研究详细介绍了一种分离腹癌细胞 (PC) 和细胞外囊泡 (EV) 来自胃癌瘤的协议. 这些组成部分对于理解瘤-肌瘤相互作用和评估治疗策略至关重要.
科学领域:
- 在瘤学瘤学.
- 细胞生物学 细胞生物学
- 生物化学 生物化学
背景情况:
- 胃癌瘤中的腹癌细胞 (PC) 和细胞外囊泡 (EV) 对于研究瘤-瘤相互作用至关重要.
- 现有的隔离这些组件的方法可以改进,以提高效率和效用.
研究的目的:
- 提出一个全面的协议,用于隔离和表征PC细胞和EVs从病人.
- 为了进一步研究这些成分在瘤微环境中的作用.
- 在此背景下,促进对新型治疗剂,如TD-139的评估.
主要方法:
- 隔离PC细胞和EVs从患者的.
- PC细胞培养和基于基醇的EV净化.
- 使用电子显微镜,纳米粒子跟踪分析和流细胞计进行表征.
- 孤立的EVs的蛋白质学概况.
- 将TD-139装入外体中进行功能测试.
主要成果:
- 从胃癌中成功分离和净化PC细胞和EVs.
- 使用多种分析技术,详细描述孤立的PC细胞和EVs.
- 演示TD-139装载到外体,以进行后续的功能评估.
- 建立一个强大的协议,研究瘤-肌相互作用在腹癌.
结论:
- 本协议提供了一种可靠的方法,用于获得胃癌研究所必需的成分.
- 这种方法支持瘤-肌瘤相互作用的研究和向治疗的开发.
- 该协议促进了对外体介导药物递送和瘤微环境的治疗调制的评估.
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